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Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation

Fainberg, Hernan P.; Birtwistle, Mark; Alagal, Reham; Alhaddad, Ahmad; Pope, Mark; Davies, Graeme; Woods, Rachel; Castellanos, Marcos; May, Sean T.; Ortori, Catharine A.; Barrett, David A.; Perry, Viv; Wiens, Frank; Stahl, Bernd; van der Beek, Eline; Sacks, Harold; Budge, Helen; Symonds, Michael E.

Authors

Mark Birtwistle

Reham Alagal

Ahmad Alhaddad

Mark Pope

Graeme Davies

Rachel Woods

Marcos Castellanos

Catharine A. Ortori

David A. Barrett

Viv Perry

Frank Wiens

Bernd Stahl

Eline van der Beek

Harold Sacks

HELEN BUDGE HELEN.BUDGE@NOTTINGHAM.AC.UK
Professor of Neonatal Medicine

Michael E. Symonds



Abstract

Brown adipose tissue (BAT) undergoes pronounced changes after birth coincident with the loss of the BAT-specifc uncoupling protein (UCP)1 and rapid fat growth. The extent to which this adaptation may vary between anatomical locations remains unknown, or whether the process is sensitive to maternal dietary supplementation. We, therefore, conducted a data mining based study on the major fat depots (i.e. epicardial, perirenal, sternal (which possess UCP1 at 7 days), subcutaneous and omental) (that do not possess UCP1) of young sheep during the frst month of life. Initially we determined what efect adding 3% canola oil to the maternal diet has on mitochondrial protein abundance in those depots which possessed UCP1. This demonstrated that maternal dietary supplementation delayed the loss of mitochondrial proteins, with the amount of cytochrome C actually being increased. Using machine learning algorithms followed by weighted gene co-expression network analysis, we demonstrated that each depot could be segregated into a unique and concise set of modules containing co-expressed genes involved in adipose function. Finally using lipidomic analysis following the maternal dietary intervention, we confrmed the perirenal depot to be most responsive. These insights point at new research avenues for examining interventions to modulate fat development in early life.

Citation

Fainberg, H. P., Birtwistle, M., Alagal, R., Alhaddad, A., Pope, M., Davies, G., …Symonds, M. E. (2018). Transcriptional analysis of adipose tissue during development reveals depot-specific responsiveness to maternal dietary supplementation. Scientific Reports, 8(1), 1-11. https://doi.org/10.1038/s41598-018-27376-3

Journal Article Type Article
Acceptance Date May 30, 2018
Online Publication Date Jun 25, 2018
Publication Date Jun 25, 2018
Deposit Date Aug 2, 2018
Publicly Available Date Aug 2, 2018
Journal Scientific Reports
Print ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 8
Issue 1
Article Number 9628
Pages 1-11
DOI https://doi.org/10.1038/s41598-018-27376-3
Public URL https://nottingham-repository.worktribe.com/output/915026
Publisher URL https://www.nature.com/articles/s41598-018-27376-3

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Transcriptional analysis of adipose tissue during development reveals depot-specifc responsiveness to maternal dietary supplementation (2.7 Mb)
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Publisher Licence URL
http://creativecommons.org/licenses/by/4.0/





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