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HAWAIIAN SKIRT controls size and floral organ number by modulating CUC1 and CUC2 expression

Gonzalez-Carranza, Zinnia H.; Zhang, Xuebin; Peters, Janny L.; Boltz, Veronique; Szecsi, Judit; Bendahmane, Mohammed; Roberts, Jeremy A.

Authors

Zinnia H. Gonzalez-Carranza

Xuebin Zhang

Janny L. Peters

Veronique Boltz

Judit Szecsi

Mohammed Bendahmane

Jeremy A. Roberts jeremy.roberts@nottingham.ac.uk



Abstract

The Arabidopsis thaliana F-box gene HAWAIIAN SKIRT (HWS) affects organ growth and the timing of floral organ abscission. The loss-of-function hws-1 mutant exhibits fused sepals and increased organ size. To understand the molecular mechanisms of HWS during plant development, we mutagenized hws-1 seeds with ethylmethylsulphonate (EMS) and screened for mutations suppressing hws-1 associated phenotypes. We isolated the shs1/hws-1 (suppressor of hws-1) mutant in which hws-1 sepal fusion phenotype was suppressed. The shs1/hws-1 mutant carries a G→A nucleotide substitution in the MIR164 binding site of CUP-SHAPED COTYLEDON 1 (CUC1) mRNA. CUC1 and CUP-SHAPED COTYLEDON 2 (CUC2) transcript levels were altered in shs1, renamed cuc1-1D, and in hws-1 mutant. Genetic interaction analyses using single, double and triple mutants of cuc1-1D, cuc2-1D (a CUC2 mutant similar to cuc1-1D), and hws-1, demonstrate that HWS, CUC1 and CUC2 act together to control floral organ number. Loss of function of HWS is associated with larger petal size due to alterations in cell proliferation and mitotic growth, a role shared with the CUC1 gene.

Journal Article Type Article
Publication Date Sep 21, 2017
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 12
Issue 9
Article Number e0185106
APA6 Citation Gonzalez-Carranza, Z. H., Zhang, X., Peters, J. L., Boltz, V., Szecsi, J., Bendahmane, M., & Roberts, J. A. (2017). HAWAIIAN SKIRT controls size and floral organ number by modulating CUC1 and CUC2 expression. PLoS ONE, 12(9), https://doi.org/10.1371/journal.pone.0185106
DOI https://doi.org/10.1371/journal.pone.0185106
Publisher URL http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185106
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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