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Nanoscale ultrasound-switchable FRET based liposomes for near-infrared fluorescence imaging in optically turbid media

Zhang, Qimei; Morgan, Stephen P.; Mather, Melissa L.

Authors

Qimei Zhang qimei.zhang@nottingham.ac.uk

Stephen P. Morgan steve.morgan@nottingham.ac.uk

Melissa L. Mather m.mather@keele.ac.uk



Abstract

A new approach to fluorescence imaging in optically turbid media centered on the use of nanoscale ultrasound-switchable FRET based liposome contrast agents is reported. Liposomes containing lipophilic carbocyanine dyes as FRET pairs with emission wavelengths located in the near-infrared window were prepared. The efficacy of FRET and self-quenching for liposomes with a range of fluorophore concentrations was first calculated from measurement of the liposome emission spectra. Exposure of the liposomes to ultrasound resulted in changes in the detected fluorescent signal, the nature of which depending on the fluorophores used, detection wavelength and the fluorophore concentration. Line scanning of a tube containing the contrast agents with 1 mm inner diameter buried at a depth of 1 cm in a heavily scattering tissue phantom demonstrated an improvement in image spatial resolution by a factor of 6.3 as compared with images obtained in the absence of ultrasound. Improvements were also seen in image contrast with the highest obtained being 9% for a liposome system containing FRET pairs. Overall the results obtained provide evidence of the potential the nanoscale ultrasound-switchable FRET based liposomes studied here have for in vivo fluorescence imaging.

Journal Article Type Article
Publication Date Sep 6, 2017
Journal Small
Print ISSN 1613-6810
Electronic ISSN 1613-6829
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 13
Issue 33
Article Number 1602895
APA6 Citation Zhang, Q., Morgan, S. P., & Mather, M. L. (2017). Nanoscale ultrasound-switchable FRET based liposomes for near-infrared fluorescence imaging in optically turbid media. Small, 13(33), doi:10.1002/smll.201602895
DOI https://doi.org/10.1002/smll.201602895
Keywords FRET; liposome; nanoscale; near-infrared; ultrasound switchable fluorescence tomography
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/smll.201602895/abstract;jsessionid=5D8A8F2BC4480655EEE4D71CF5BC557F.f03t01
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This is the peer reviewed version of the following article: Nanoscale ultrasound-switchable FRET based liposomes for near-infrared fluorescence imaging in optically turbid media. Qimei Zhang, Stephen P. Morgan, and Melissa L. Mather, Small, 13 (2017), 1602895 which has been published in final form at doi:10.1002/smll.201602895. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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