A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore

Brandenburger, Eileen; Gressler, Markus; Leonhardt, Robin; Lackner, Gerald; Habel, Andreas; Hertweck, Christian; Brock, Matthias; Hoffmeister, Dirk

Abstract

The model white-rot basidiomycete Ceriporiopsis (Gelatoporia) subvermispora B encodes putative natural product biosynthesis genes. Among them is the gene for the seven-domain nonribosomal peptide synthetase CsNPS2. It is a member of the as-yet uncharacterized fungal type VI siderophore synthetase family which is highly conserved and widely distributed among the basidiomycetes. These enzymes include only one adenylation (A) domain, i.e., one complete peptide synthetase module and two thiolation/condensation (T-C) di-domain partial modules which, together, constitute an AT1C1T2C2T3C3 domain setup. The full-length CsNPS2 enzyme (274.5 kDa) was heterologously produced as polyhistidine fusion in Aspergillus niger as soluble and active protein. N5-acetyl-N5-hydroxy-L-ornithine (L-AHO) and N5-cis anhydromevalonyl-N5-hydroxy-L-ornithine (L-AMHO) were accepted as substrates, as assessed in vitro using the substrate-dependent [32P] ATP-pyrophosphate radioisotope exchange assay. Full-length holo-CsNPS2 catalyzed amide bond formation between three L-AHO molecules to release the linear L-AHO trimer, called basidioferrin, as product in vitro, which was verified by LC-HRESIMS. Phylogenetic analyses suggest that type VI family siderophore synthetases are widespread in mushrooms and have evolved in a common ancestor of basidiomycetes.

Citation

Brandenburger, E., Gressler, M., Leonhardt, R., Lackner, G., Habel, A., Hertweck, C., …Hoffmeister, D. (in press). A highly conserved basidiomycete peptide synthetase produces a trimeric hydroxamate siderophore. Applied and Environmental Microbiology, https://doi.org/10.1128/AEM.01478-17

• Journal Article Type: Article
• Acceptance Date: Aug 18, 2017
• Online Publication Date: Aug 25, 2017
• Deposit Date: Sep 7, 2017
• Publicly Available Date: Aug 26, 2018
• Journal: Applied and Environmental Microbiology
• Print ISSN: 0099-2240
• Electronic ISSN: 1098-5336
• Publisher: American Society for Microbiology
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1128/AEM.01478-17
• Public URL: https://nottingham-repository.worktribe.com/output/879180
• Publisher URL: http://aem.asm.org/content/early/2017/08/21/AEM.01478-17
• Contract Date: Sep 7, 2017