A rapid IL-17 response to Cryptosporidium parvum in the bovine intestine

Drinkall, Emma; Wass, Matthew J.; Coffey, Tracey J.; Flynn, Robin J.

Abstract

Cryptosporidium parvum causes diarrhoea, due to villi damage, in livestock and humans globally. Immunity develops after repeated infections but initial infections can be severe, highlighting the importance of early infection dynamics. We have modelled early C. parvum infection in bovine jejunum biopsies. IL-17A accumulated over time peaking at 9 h post-infection, with no effect of infection on IL-1β; antibiotics positively influenced IL-17A as higher levels were found in cultures with antibiotics. Infection of primary fibroblasts resulted in lower plaque formation when fibroblasts were primed with IL-17A. Our results indicate a role for IL-17A in reducing C. parvum-dependent host cell damage.

Citation

Drinkall, E., Wass, M. J., Coffey, T. J., & Flynn, R. J. (in press). A rapid IL-17 response to Cryptosporidium parvum in the bovine intestine. Veterinary Immunology and Immunopathology, 191, https://doi.org/10.1016/j.vetimm.2017.07.009

• Journal Article Type: Article
• Acceptance Date: Jul 18, 2017
• Online Publication Date: Jul 23, 2017
• Deposit Date: Jul 27, 2017
• Publicly Available Date: Jul 27, 2017
• Journal: Veterinary Immunology and Immunopathology
• Print ISSN: 0165-2427
• Electronic ISSN: 1873-2534
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 191
• DOI: https://doi.org/10.1016/j.vetimm.2017.07.009
• Keywords: Cryptosporidium parvum; Biopsy; IL-17A; Intestine; Villi; Bovine
• Public URL: https://nottingham-repository.worktribe.com/output/873775
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0165242717302945
• Contract Date: Jul 27, 2017