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Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy

Penon, Oriol; Marín, María J.; Russell, David A.; Pérez-García, Lluïsa

Authors

Oriol Penon

María J. Marín

David A. Russell

Lluïsa Pérez-García Lluisa.PerezGarcia1@nottingham.ac.uk



Abstract

Photodynamic therapy (PDT) is a treatment of cancer by which tumour cells are destroyed using reactive oxygen species produced by photosensitizers following activation with visible or near infrared light. Successful PDT depends on the solubility and the targeting ability of the photosensitizers. In this work, the synthesis of a porphyrin-based water soluble nanoparticle conjugate containing a targeting agent that recognizes the erbB2 receptor overexpressed on the surface of particular cancer cells is reported. The nanoparticle conjugates were synthesized following two different protocols, viz. a biphasic and a monophasic method, with the aim to determine which method yielded the optimal nanosystem for potential PDT applications. The nanoparticles were characterized using UV–Vis absorption and fluorescence spectroscopies together with transmission electron microscopy and zeta potential measurements; and their ability to produce singlet oxygen following irradiation was investigated following the decay in absorption of a singlet oxygen probe. The nanoparticles synthesized using the monophasic method were shown to produce the highest amount of singlet oxygen and were further functionalized with anti-erbB2 antibody to target the erbB2 receptors expressed on the surface of SK-BR-3 human breast cancer cells. The water soluble, antibody-porphyrin nanoparticle conjugates were shown to elicit targeted PDT of the breast cancer cells.

Journal Article Type Article
Publication Date Jun 15, 2017
Journal Journal of Colloid and Interface Science
Print ISSN 0021-9797
Electronic ISSN 1095-7103
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 496
APA6 Citation Penon, O., Marín, M. J., Russell, D. A., & Pérez-García, L. (2017). Water soluble, multifunctional antibody-porphyrin gold nanoparticles for targeted photodynamic therapy. Journal of Colloid and Interface Science, 496, doi:10.1016/j.jcis.2017.02.006
DOI https://doi.org/10.1016/j.jcis.2017.02.006
Keywords Photodynamic therapy ; Multifunctional nanoparticles ; Porphyrins ; Gold nanoparticles (AuNPs) ; Water soluble nanoconjugates ; Targeting antibodies ; Singlet oxygen production ; In vitro phototoxicity ; Human breast cancer cells
Publisher URL https://www.sciencedirect.com/science/article/pii/S002197971730156X?via%3Dihub
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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