Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken

Barbosa, Fernanda de Oliveira; Freitas Neto, Oliveiro Caetano de; Batista, Diego Felipe Alves; Almeida, Adriana Maria de; Rubio, Marcela da Silva; Alves, Lucas Bocchini Rodrigues; Vasconcelos, Rosemeire de Oliveira; Barrow, Paul Andrew; Berchieri Junior, Angelo

Abstract

Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ?motB) or non-flagellated (SE ?fliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ?motB and SE ?fliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE ?motB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE ?fliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken.

Citation

Barbosa, F. D. O., Freitas Neto, O. C. D., Batista, D. F. A., Almeida, A. M. D., Rubio, M. D. S., Alves, L. B. R., …Berchieri Junior, A. (in press). Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken. Brazilian Journal of Microbiology, https://doi.org/10.1016/j.bjm.2017.01.012

• Journal Article Type: Article
• Acceptance Date: Jan 20, 2017
• Online Publication Date: Jun 7, 2017
• Deposit Date: Aug 23, 2017
• Publicly Available Date: Aug 23, 2017
• Journal: Brazilian Journal of Microbiology
• Electronic ISSN: 1517-8382
• Peer Reviewed: Peer Reviewed
• DOI: https://doi.org/10.1016/j.bjm.2017.01.012
• Keywords: Mutation ; Flagellum ; Virulence factors ; Poultry ; Gut colonisation
• Public URL: https://nottingham-repository.worktribe.com/output/864522
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S1517838216307080?via%3Dihub