A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity

Afonso, Maria M.; Pinchbeck, Gina L.; Smith, Shirley L.; Daly, Janet M.; Gaskell, Rosalind M.; Dawson, Susan; Radford, Alan D.

Abstract

Background

Feline calicivirus (FCV) is an important pathogen of cats for which vaccination is regularly practised. Long-term use of established vaccine antigens raises the theoretical possibility that field viruses could become resistant. This study aimed to assess the current ability of the FCV-F9 vaccine strain to neutralise a randomly collected contemporary panel of FCV field strains collected prospectively in six European countries.
Methods

Veterinary practices (64) were randomly selected from six countries (UK, Sweden, Netherlands, Germany, France and Italy). Oropharyngeal swabs were requested from 30 (UK) and 40 (other countries) cats attending each practice. Presence of FCV was determined by virus isolation, and risk factors for FCV shedding assessed by multivariable logistic regression. Phylogenetic analyses were used to describe the FCV population structure. In vitro virus neutralisation assays were performed to evaluate FCV-F9 cross-reactivity using plasma from four vaccinated cats.
Results

The overall prevalence of FCV was 9.2%. Risk factors positively associated with FCV shedding included multi-cat households, chronic gingivostomatitis, younger age, not being neutered, as well as residing in certain countries. Phylogenetic analysis showed extensive variability and no countrywide clusters. Despite being first isolated in the 1950s, FCV-F9 clustered with contemporary field isolates. Plasma raised to FCV-F9 neutralized 97% of tested isolates (titres 1:4 to 1:5792), with 26.5%, 35.7% and 50% of isolates being neutralized by 5, 10 and 20 antibody units respectively.
Conclusions

This study represents the largest prospective analysis of FCV diversity and antigenic cross-reactivity at a European level. The scale and random nature of sampling used gives confidence that the FCV isolates used are broadly representative of FCVs that cats are exposed to in these countries. The in vitro neutralisation results suggest that antibodies raised to FCV-F9 remain broadly cross-reactive to contemporary FCV isolates across the European countries sampled.

Citation

Afonso, M. M., Pinchbeck, G. L., Smith, S. L., Daly, J. M., Gaskell, R. M., Dawson, S., & Radford, A. D. (2017). A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity. Vaccine, 35(20), 2753-2760. https://doi.org/10.1016/j.vaccine.2017.03.030

• Journal Article Type: Article
• Acceptance Date: Mar 4, 2017
• Online Publication Date: Apr 5, 2017
• Publication Date: May 9, 2017
• Deposit Date: Apr 7, 2017
• Publicly Available Date: Apr 7, 2017
• Journal: Vaccine
• Print ISSN: 0264-410X
• Electronic ISSN: 1873-2518
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 35
• Issue: 20
• Pages: 2753-2760
• DOI: https://doi.org/10.1016/j.vaccine.2017.03.030
• Keywords: Feline calicivirus; Vaccine; Neutralisation; Phylogeny; Epidemiology
• Public URL: https://nottingham-repository.worktribe.com/output/859986
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0264410X17303365
• Additional Information: This article is maintained by: Elsevier; Article Title: A multi-national European cross-sectional study of feline calicivirus epidemiology, diversity and vaccine cross-reactivity; Journal Title: Vaccine; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.vaccine.2017.03.030; Content Type: article; Copyright: © 2017 The Authors. Published by Elsevier Ltd.
• Contract Date: Apr 7, 2017