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Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity

Pickles, S.F.; Pritchard, David I.

Authors

S.F. Pickles samantha.pickles@nottingham.ac.uk

David I. Pritchard david.pritchard@nottingham.ac.uk



Abstract

Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by the larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, we have developed a complementary radial-diffusion enzymatic assay (RDEA) to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardised conditions, against a gelatin substrate. Using basic laboratory equipment and reagents, the assay is rapid and suited to high-throughput. Assay reproducibility is high (SD 0.06 - 0.27, CV 0.75 - 4.31%) and the LAP collection procedure does not adversely affect larval survival (mortality < 2%). As a cost- and time-effective method, it is suited to academic or industrial use, supporting good manufacturing (or laboratory) practice (GMP and GLP) as a quality control (QC) assay.

Journal Article Type Article
Publication Date Apr 25, 2017
Journal Medical and Veterinary Entomology
Print ISSN 0269-283X
Electronic ISSN 1365-2915
Publisher Wiley
Peer Reviewed Not Peer Reviewed
Volume 31
Issue 2
APA6 Citation Pickles, S., & Pritchard, D. I. (2017). Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Medical and Veterinary Entomology, 31(2), doi:10.1111/mve.12220
DOI https://doi.org/10.1111/mve.12220
Keywords Lucilia sericata; Debridement; Gelatinase; Medical device; Quality control; Wound management
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/mve.12220/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This is the pre-peer reviewed version of the following article: PICKLES, S. F. and PRITCHARD, D. I. (2017), Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Med Vet Entomol, 31: 200–206. doi:10.1111/mve.12220, which has been published in final form at http://onlinelibrary.wi...1111/mve.12220/abstract This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

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RDEA QC for ePrints .pdf (809 Kb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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