S.F. Pickles
Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity
Pickles, S.F.; Pritchard, David I.
Authors
David I. Pritchard
Abstract
Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by the larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, we have developed a complementary radial-diffusion enzymatic assay (RDEA) to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardised conditions, against a gelatin substrate. Using basic laboratory equipment and reagents, the assay is rapid and suited to high-throughput. Assay reproducibility is high (SD 0.06 - 0.27, CV 0.75 - 4.31%) and the LAP collection procedure does not adversely affect larval survival (mortality < 2%). As a cost- and time-effective method, it is suited to academic or industrial use, supporting good manufacturing (or laboratory) practice (GMP and GLP) as a quality control (QC) assay.
Citation
Pickles, S., & Pritchard, D. I. (2017). Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Medical and Veterinary Entomology, 31(2), 200-206. https://doi.org/10.1111/mve.12220
Journal Article Type | Article |
---|---|
Acceptance Date | Nov 13, 2016 |
Online Publication Date | Jan 24, 2017 |
Publication Date | Apr 25, 2017 |
Deposit Date | Oct 18, 2017 |
Publicly Available Date | Oct 18, 2017 |
Journal | Medical and Veterinary Entomology |
Print ISSN | 0269-283X |
Electronic ISSN | 1365-2915 |
Publisher | Wiley |
Peer Reviewed | Not Peer Reviewed |
Volume | 31 |
Issue | 2 |
Pages | 200-206 |
DOI | https://doi.org/10.1111/mve.12220 |
Keywords | Lucilia sericata; Debridement; Gelatinase; Medical device; Quality control; Wound management |
Public URL | https://nottingham-repository.worktribe.com/output/857553 |
Publisher URL | http://onlinelibrary.wiley.com/doi/10.1111/mve.12220/abstract |
Additional Information | This is the pre-peer reviewed version of the following article: PICKLES, S. F. and PRITCHARD, D. I. (2017), Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Med Vet Entomol, 31: 200–206. doi:10.1111/mve.12220, which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/mve.12220/abstract This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving. |
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