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Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity

Pickles, S.F.; Pritchard, David I.

Authors

S.F. Pickles samantha.pickles@nottingham.ac.uk

David I. Pritchard david.pritchard@nottingham.ac.uk



Abstract

Lucilia sericata Meigen (Diptera: Calliphoridae) larvae are manufactured worldwide for the treatment of chronic wounds. Published research has confirmed that the primary clinical effect of the product, debridement (the degradation of non-viable wound tissue), is accomplished by a range of enzymes released by the larvae during feeding. The quality assessment of larval activity is currently achieved during production using meat-based assays, which monitor insect growth and/or the reduction in substrate mass. To support this, we have developed a complementary radial-diffusion enzymatic assay (RDEA) to produce a visual and measureable indication of the activity of larval alimentary products (LAP) collected under standardised conditions, against a gelatin substrate. Using basic laboratory equipment and reagents, the assay is rapid and suited to high-throughput. Assay reproducibility is high (SD 0.06 - 0.27, CV 0.75 - 4.31%) and the LAP collection procedure does not adversely affect larval survival (mortality < 2%). As a cost- and time-effective method, it is suited to academic or industrial use, supporting good manufacturing (or laboratory) practice (GMP and GLP) as a quality control (QC) assay.

Citation

Pickles, S., & Pritchard, D. I. (2017). Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Medical and Veterinary Entomology, 31(2), doi:10.1111/mve.12220

Journal Article Type Article
Acceptance Date Nov 13, 2016
Online Publication Date Jan 24, 2017
Publication Date Apr 25, 2017
Deposit Date Oct 18, 2017
Publicly Available Date Oct 18, 2017
Journal Medical and Veterinary Entomology
Print ISSN 0269-283X
Electronic ISSN 1365-2915
Publisher Wiley
Peer Reviewed Not Peer Reviewed
Volume 31
Issue 2
DOI https://doi.org/10.1111/mve.12220
Keywords Lucilia sericata; Debridement; Gelatinase; Medical device; Quality control; Wound management
Public URL http://eprints.nottingham.ac.uk/id/eprint/47337
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/mve.12220/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This is the pre-peer reviewed version of the following article: PICKLES, S. F. and PRITCHARD, D. I. (2017), Quality control of a medicinal larval (Lucilia sericata) debridement device based on released gelatinase activity. Med Vet Entomol, 31: 200–206. doi:10.1111/mve.12220, which has been published in final form at http://onlinelibrary.wi...1111/mve.12220/abstract This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.

Files

RDEA QC for ePrints .pdf (809 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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