Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells

Xu, Sha-Sha; Liu, Qing-Mei; Xiao, An-Feng; Maleki, Soheila J.; Alcocer, Marcos; Gao, Yuan-Yuan; Cao, Min-Jie; Liu, Guang-Ming

Abstract

In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77.7%. In addition, the production of interleukin (IL)-4 and IL-13 was diminished in the ESO groups compared to the non-ESO-treated group. Furthermore, ESO could up-regulate Treg cells by 22.2?97.1%. In conclusion, ESO decreased the allergy response in mice by reducing basophil degranulation, up-regulating Treg cells via Forkhead box protein 3 (Foxp3), and releasing IL-10. ESO may have preventive and therapeutic potential in allergic disease.

Citation

Xu, S., Liu, Q., Xiao, A., Maleki, S. J., Alcocer, M., Gao, Y., …Liu, G. (2017). Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells. Journal of Agricultural and Food Chemistry, 65(15), https://doi.org/10.1021/acs.jafc.7b00389

• Journal Article Type: Article
• Acceptance Date: Mar 30, 2017
• Publication Date: Mar 30, 2017
• Deposit Date: Sep 6, 2017
• Journal: Journal of Agricultral and Food Chemistry
• Print ISSN: 0021-8561
• Electronic ISSN: 1520-5118
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 65
• Issue: 15
• DOI: https://doi.org/10.1021/acs.jafc.7b00389
• Keywords: Eucheuma cottonii sulfated oligosaccharide (ESO); food allergy; mast cells; murine model; Treg cells
• Public URL: https://nottingham-repository.worktribe.com/output/853470
• Publisher URL: http://pubs.acs.org/doi/abs/10.1021/acs.jafc.7b00389