Negative regulation of violacein biosynthesis in Chromobacterium violaceum

Devescovi, Giulia; Kojic, Milan; Covaceuszach, Sonia; C�mara, Miguel; Williams, Paul; Bertani, Iris; Subramoni, Sujatha; Venturi, Vittorio

doi:10.3389/fmicb.2017.00349

Negative regulation of violacein biosynthesis in Chromobacterium violaceum

Devescovi, Giulia; Kojic, Milan; Covaceuszach, Sonia; C�mara, Miguel; Williams, Paul; Bertani, Iris; Subramoni, Sujatha; Venturi, Vittorio

Authors

Giulia Devescovi

Milan Kojic

Sonia Covaceuszach

Miguel C�mara

PAUL WILLIAMS PAUL.WILLIAMS@NOTTINGHAM.AC.UK
Professor of Molecular Microbiology

Iris Bertani

Sujatha Subramoni

Vittorio Venturi

Abstract

In Chromobacteium violaceum, the purple pigment violacein is under positive regulation by the N-acylhomoserine lactone CviI/R quorum sensing system and negative regulation by an uncharacterized putative repressor. In this study we report that the biosynthesis of violacein is negatively controlled by a novel repressor protein, VioS. The violacein operon is regulated negatively by VioS and positively by the CviI/R system in both C. violaceum and in a heterologous Escherichia coli genetic background. VioS does not regulate the CviI/R system and apart from violacein, VioS and quorum sensing regulate other phenotypes antagonistically. Quorum sensing regulated phenotypes in C. violaceum are therefore further regulated providing an additional level of control.

Citation

Devescovi, G., Kojic, M., Covaceuszach, S., Cámara, M., Williams, P., Bertani, I., …Venturi, V. (in press). Negative regulation of violacein biosynthesis in Chromobacterium violaceum. Frontiers in Microbiology, 8, Article 349. https://doi.org/10.3389/fmicb.2017.00349

Journal Article Type	Article
Acceptance Date	Feb 20, 2017
Online Publication Date	Feb 20, 2017
Deposit Date	Feb 20, 2017
Publicly Available Date	Feb 20, 2017
Journal	Frontiers in Microbiology
Electronic ISSN	1664-302X
Publisher	Frontiers Media
Peer Reviewed	Peer Reviewed
Volume	8
Article Number	349
DOI	https://doi.org/10.3389/fmicb.2017.00349
Keywords	Chromobacterium violaceum, VioS, CviI/R quorum sensing, regulation, Violacein, Chitinase activity, Protease activity
Public URL	https://nottingham-repository.worktribe.com/output/845155
Publisher URL	http://journal.frontiersin.org/article/10.3389/fmicb.2017.00349/abstract
Contract Date	Feb 20, 2017