Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model

Geng, Shizhong; Tian, Qin; Guo, Rongxian; Jiao, Yang; Barrow, Paul; Yin, Chao; Wang, Yaonan; Geng, Haopeng; Pan, Zhiming; Jiao, Xinan

Abstract

A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were involved in invasion, cell division, metabolism and bacterial defence. The competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains confirmed their attenuation.

Citation

Geng, S., Tian, Q., Guo, R., Jiao, Y., Barrow, P., Yin, C., …Jiao, X. (2017). Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model. Microbial Pathogenesis, 104, https://doi.org/10.1016/j.micpath.2017.01.014

• Journal Article Type: Article
• Acceptance Date: Jan 5, 2017
• Online Publication Date: Jan 12, 2017
• Publication Date: Mar 1, 2017
• Deposit Date: Aug 23, 2017
• Publicly Available Date: Aug 23, 2017
• Journal: Microbial Pathogenesis
• Print ISSN: 0882-4010
• Electronic ISSN: 1096-1208
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 104
• DOI: https://doi.org/10.1016/j.micpath.2017.01.014
• Keywords: Salmonella Pullorum ; Signature-tagged mutagenesis ; Chicken embryo
• Public URL: https://nottingham-repository.worktribe.com/output/842775
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0882401016302704?via%3Dihub