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Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model

Geng, Shizhong; Tian, Qin; Guo, Rongxian; Jiao, Yang; Barrow, Paul; Yin, Chao; Wang, Yaonan; Geng, Haopeng; Pan, Zhiming; Jiao, Xinan

Authors

Shizhong Geng

Qin Tian

Rongxian Guo

Yang Jiao

Paul Barrow

Chao Yin

Yaonan Wang

Haopeng Geng

Zhiming Pan

Xinan Jiao



Abstract

A key feature of the fowl-specific pathogen Salmonella Pullorum is its vertical transmission to progeny via the egg. In this study, PCR signature-tagged mutagenesis identified nine genes of a strain of S. Pullorum that contributed to survival in the chicken embryo during incubation. The genes were involved in invasion, cell division, metabolism and bacterial defence. The competition index in vivo and in vitro together with a virulence evaluation for chicken embryos of all nine mutant strains confirmed their attenuation.

Journal Article Type Article
Publication Date Mar 1, 2017
Journal Microbial Pathogenesis
Print ISSN 0882-4010
Electronic ISSN 1096-1208
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 104
APA6 Citation Geng, S., Tian, Q., Guo, R., Jiao, Y., Barrow, P., Yin, C., …Jiao, X. (2017). Identification by PCR signature-tagged mutagenesis of attenuated Salmonella Pullorum mutants and corresponding genes in a chicken embryo model. Microbial Pathogenesis, 104, doi:10.1016/j.micpath.2017.01.014
DOI https://doi.org/10.1016/j.micpath.2017.01.014
Keywords Salmonella Pullorum ; Signature-tagged mutagenesis ; Chicken embryo
Publisher URL http://www.sciencedirect.com/science/article/pii/S0882401016302704?via%3Dihub
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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