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Disruption of diphenylalanine assembly by a Boc-modified variant

Creasey, Rhiannon; Louzao, Iria; Arnon, Zohar; Marco, Pini; Adler-Abramovich, Lihi; Roberts, Clive J.; Gazit, Ehud; Tendler, Saul J.B.

Authors

Rhiannon Creasey

Iria Louzao

Zohar Arnon

Pini Marco

Lihi Adler-Abramovich

Ehud Gazit

Saul J.B. Tendler



Abstract

Peptide-based biomaterials are key to the future of diagnostics and therapy, promoting applications such as tissue scaffolds and drug delivery vehicles. To realise the full potential of the peptide systems, control and optimisation of material properties are essential. Here we invesigated the co-assembly of the minimal amyloid motif peptide, diphenylalanine (FF), and its tert-butoxycarbonyl (Boc)-modified derivative. Using Atomic Force Microscopy, we demonstrated that the co-assembled fibers are less rigid and show a curvier morphology. We propose that the Boc-modification of FF disrupts the hydrogen bond packing of adjacent N-termini, as supported by Fourier transform infrared and fluorescence spectroscopic data. Such rationally modified co-assemblies offer chemical functionality for after-assembly modification and controllable surface properties for tissue engineering scaffolds, along with tunable morphological vs. mechanical properties.

Citation

Creasey, R., Louzao, I., Arnon, Z., Marco, P., Adler-Abramovich, L., Roberts, C. J., …Tendler, S. J. (2016). Disruption of diphenylalanine assembly by a Boc-modified variant. Soft Matter, 12(47), 9451-9457. https://doi.org/10.1039/c6sm01770c

Journal Article Type Article
Acceptance Date Nov 8, 2016
Online Publication Date Nov 8, 2016
Publication Date Nov 8, 2016
Deposit Date May 18, 2017
Publicly Available Date May 18, 2017
Journal Soft Matter
Print ISSN 1744-683X
Electronic ISSN 1744-6848
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 12
Issue 47
Pages 9451-9457
DOI https://doi.org/10.1039/c6sm01770c
Public URL https://nottingham-repository.worktribe.com/output/829583
Publisher URL http://pubs.rsc.org/en/content/articlelanding/2016/sm/c6sm01770c#!divAbstract
Additional Information : This document is CrossCheck deposited; : Supplementary Information; : Rhiannon C. G. Creasey (ORCID); : Rhiannon C. G. Creasey (ResearcherID); : The Royal Society of Chemistry has an exclusive publication licence for this journal; : Received 2 August 2016; Accepted 8 November 2016; Accepted Manuscript published 8 November 2016; Advance Article published 14 November 2016; Version of Record published 28 November 2016
Contract Date May 18, 2017

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