Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection

Stevenson, Emma C.; Major, Giles A.; Spiller, Robin C.; Kuehne, Sarah A.; Minton, Nigel P.

doi:10.1128/jcm.01025-16

Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection

Stevenson, Emma C.; Major, Giles A.; Spiller, Robin C.; Kuehne, Sarah A.; Minton, Nigel P.

Authors

Emma C. Stevenson

Giles A. Major

ROBIN SPILLER ROBIN.SPILLER@NOTTINGHAM.AC.UK
Professor of Gastroenterology

Sarah A. Kuehne

Professor NIGEL MINTON nigel.minton@nottingham.ac.uk
Professor of Applied Molecular Microbiology

Contributors

B. A. Forbes
Editor

Abstract

Clostridium difficile (Peptoclostridium difficile) is a common health care-associated infection with a disproportionately high incidence in elderly patients. Disease symptoms range from mild diarrhea to life-threatening pseudomembranous colitis. Around 20% of patients may suffer recurrent disease, which often requires rehospitalization of patients. C. difficile was isolated from stool samples from a patient with two recurrent C. difficile infections. PCR ribotyping, whole-genome sequencing, and phenotypic assays were used to characterize these isolates. Genotypic and phenotypic screening of C. difficile isolates revealed multiple PCR ribotypes present and the emergence of rifamycin resistance during the infection cycle. Understanding both the clinical and bacterial factors that contribute to the course of recurrent infection could inform strategies to reduce recurrence. (This study has been registered at ClinicalTrials.gov under registration no. NCT01670149.)

Citation

Stevenson, E. C., Major, G. A., Spiller, R. C., Kuehne, S. A., & Minton, N. P. (2016). Coinfection and Emergence of Rifamycin Resistance during a Recurrent Clostridium difficile Infection. Journal of Clinical Microbiology, 54(11), 2689-2694. https://doi.org/10.1128/jcm.01025-16

Journal Article Type	Article
Acceptance Date	Aug 15, 2016
Online Publication Date	Aug 24, 2016
Publication Date	Nov 15, 2016
Deposit Date	Oct 4, 2016
Publicly Available Date	Oct 4, 2016
Journal	Journal of Clinical Microbiology
Print ISSN	0095-1137
Electronic ISSN	1098-660X
Publisher	American Society for Microbiology
Peer Reviewed	Peer Reviewed
Volume	54
Issue	11
Pages	2689-2694
DOI	https://doi.org/10.1128/jcm.01025-16
Public URL	https://nottingham-repository.worktribe.com/output/828620
Publisher URL	http://jcm.asm.org/content/54/11/2689