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Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis

Harding, Stephen E.; Gillis, Richard B.; Adams, Gary G.

Assessing sedimentation equilibrium profiles in analytical ultracentrifugation experiments on macromolecules: from simple average molecular weight analysis to molecular weight distribution and interaction analysis Thumbnail


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STEPHEN HARDING STEVE.HARDING@NOTTINGHAM.AC.UK
Professor of Applied Biochemistry

Richard B. Gillis

Gary G. Adams



Abstract

Molecular weights (molar masses), molecular weight distributions, dissociation constants and other interaction parameters are fundamental characteristics of proteins, nucleic acids, polysaccharides and glycoconjugates in solution. Sedimentation equilibrium in the analytical ultracentrifugation provides a powerful method with no supplementary immobilization, columns or membranes required. It is particularly powerful when used in conjunction with its sister technique, namely sedimentation velocity analysis. We describe key approaches now available and their application to the characterisation of antibodies polysaccharides and glycoconjugates. We indicate how major complications such as thermodynamic non-ideality can now be routinely dealt with, thanks to a great extent to the extensive contribution of Professor DonWinzor over several decades of research.

Journal Article Type Article
Acceptance Date Oct 6, 2016
Online Publication Date Nov 22, 2016
Deposit Date Nov 1, 2016
Publicly Available Date Nov 22, 2016
Journal Biophysical Reviews
Print ISSN 1867-2450
Electronic ISSN 1867-2469
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 8
Issue 4
DOI https://doi.org/10.1007/s12551-016-0232-8
Keywords SEDFIT-MSTAR; non-ideality; extended fujita; COVOL
Public URL https://nottingham-repository.worktribe.com/output/827380
Publisher URL https://link.springer.com/article/10.1007%2Fs12551-016-0232-8

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