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Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants

Contente, Martina Letizia; Planchestainer, Matteo; Molinari, Francesco; Paradisi, Francesca

Authors

Martina Letizia Contente

Matteo Planchestainer

Francesco Molinari



Abstract

A transaminase from Halomonas elongata and four mutants generated by an in silico-based design, were recombinantly produced in E. coli, purified and applied to the amination of mono-substituted aromatic carbonyl-derivatives. While benzaldehyde derivatives resulted excellent substrates, only NO2-acetophenones were transformed into the (S)-amine with high enantioselectivity. The different behaviour of wild-type and mutated transaminases was assessed by in silico substrate binding mode studies.

Citation

Contente, M. L., Planchestainer, M., Molinari, F., & Paradisi, F. (in press). Stereoelectronic effects in the reaction of aromatic substrates catalysed by Halomonas elongata transaminase and its mutants. Organic and Biomolecular Chemistry, 14, https://doi.org/10.1039/C6OB01629D

Journal Article Type Article
Acceptance Date Sep 6, 2016
Online Publication Date Sep 6, 2016
Deposit Date Nov 29, 2016
Publicly Available Date Nov 29, 2016
Journal Organic and Biomolecular Chemistry
Print ISSN 1477-0520
Electronic ISSN 1477-0539
Publisher Royal Society of Chemistry
Peer Reviewed Peer Reviewed
Volume 14
DOI https://doi.org/10.1039/C6OB01629D
Public URL http://eprints.nottingham.ac.uk/id/eprint/38985
Publisher URL http://pubs.rsc.org/en/Content/ArticleLanding/2016/OB/C6OB01629D#!divAbstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

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2016 OBC repository.pdf (639 Kb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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