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An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms

Harrison, Freya; Diggle, Stephen P.

Authors

Freya Harrison

Stephen P. Diggle



Abstract

A key aim in microbiology is to determine the genetic and phenotypic bases of bacterial virulence, persistence and antimicrobial resistance in chronic biofilm infections. This requires tractable, high-throughput models that reflect the physical and chemical environment encountered in specific infection contexts. Such models will increase the predictive power of microbiological experiments and provide platforms for enhanced testing of novel antibacterial or antivirulence therapies. We present an optimised ex vivo model of cystic fibrosis lung infection: ex vivo culture of pig bronchiolar tissue in artificial cystic fibrosis mucus. We focus on the formation of biofilms by Pseudomonas aeruginosa. We show highly repeatable and specific formation of biofilms that resemble clinical biofilms by a commonly-studied lab strain and ten cystic fibrosis isolates of this key opportunistic pathogen.

Citation

Harrison, F., & Diggle, S. P. (in press). An ex vivo lung model to study bronchioles infected with Pseudomonas aeruginosa biofilms. Microbiology, 162, https://doi.org/10.1099/mic.0.000352

Journal Article Type Article
Acceptance Date Aug 9, 2016
Online Publication Date Oct 1, 2016
Deposit Date Aug 23, 2016
Publicly Available Date Mar 28, 2024
Journal Microbiology
Electronic ISSN 1465-2080
Publisher Microbiology Society
Peer Reviewed Peer Reviewed
Volume 162
DOI https://doi.org/10.1099/mic.0.000352
Keywords Biofilm, 3Rs, Chronic infection, Cystic fibrosis, Respiratory infection, Pseudomonas aeruginosa
Public URL https://nottingham-repository.worktribe.com/output/807903
Publisher URL http://mic.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000352

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