Identifying polymorphisms in the Alzheimer's related APP gene using the MinION sequencer

Brookes, Keeley

Abstract

The MinION is a bench top sequencer by Oxford nanopore technologies (ONT) that allows long reads of DNA sequence. Few studies have tested whether polymorphisms can be detected using this device. Several polymorphisms within the APP gene were used to test this capability. Library preparation and sequencing were performed using standard ONT protocols for samples harbouring ive different mutations. Alignments to the reference sequence were analysed in MinoTour and basecalls were manually investigated using proportion of reference calls between samples to identify the variants. MinoTour’s algorithm for variant detection was unable to identify the polymorphisms due to high base calling error rate. By calculating the difference in reference basecall proportions along the amplicon, it was possible to identify the polymorphisms above a Bonferroni-corrected threshold (p

Citation

Brookes, K. (2016). Identifying polymorphisms in the Alzheimer's related APP gene using the MinION sequencer. https://doi.org/10.4172/jngsa.1000125

• Journal Article Type: Article
• Acceptance Date: May 11, 2016
• Publication Date: May 13, 2016
• Deposit Date: May 27, 2016
• Publicly Available Date: May 27, 2016
• Journal: Journal of Next Generation Sequencing & Applications
• Electronic ISSN: 2469-9853
• Publisher: OMICS International
• Peer Reviewed: Peer Reviewed
• Volume: 3
• Issue: 1
• DOI: https://doi.org/10.4172/jngsa.1000125
• Keywords: Nanopore technology; MinION; Sequencing; Polymorphism detection; Deletion
• Public URL: https://nottingham-repository.worktribe.com/output/790070