Charlotte G. Sinnett
Helicobacter pylori vacA transcription is genetically-determined and stratifies the level of human gastric inflammation and atrophy
Sinnett, Charlotte G.; Letley, Darren P.; Narayana, Geetha L.; Patel, Sapna Rohitbhai; Hussein, Nawfal Rasheed; Zaitoun, Abed M.; Robinson, Karen; Atherton, John C.
Authors
Darren P. Letley
Geetha L. Narayana
Sapna Rohitbhai Patel
Nawfal Rasheed Hussein
Abed M. Zaitoun
KAREN ROBINSON karen.robinson@nottingham.ac.uk
Professor in Gastrointestinal Infection and Immunity
John C. Atherton
Abstract
Aims Helicobacter pylori infection is the major cause of peptic ulceration and gastric cancer, and an important virulence determinant is its vacuolating cytotoxin, vacA. Previously, we have described allelic variation in vacA which determines toxin activity and disease risk. We now aimed to quantify vacA mRNA expression in the human stomach, define its genetic determinants and assess how well it predicted gastric pathology. Methods Gastric biopsies were donated by 39 H. pylori-infected patients attending for endoscopy at Queen’s Medical Centre, Nottingham, UK. Total RNA was extracted, and vacA mRNA quantified by reverse transcriptase quantitative polymerase chain reaction. Separate biopsies were histologically scored for inflammation and atrophy using the updated Sydney system. H. pylori strains were isolated from further biopsies, and the nucleotide sequence upstream of vacA determined. Results vacA mRNA levels in human stomachs varied by two orders of magnitude independently of vacA allelic type. Among vacA i1-type (toxic) strains, increased vacA expression was strongly associated with higher grade gastric inflammation (p
Citation
Sinnett, C. G., Letley, D. P., Narayana, G. L., Patel, S. R., Hussein, N. R., Zaitoun, A. M., …Atherton, J. C. (2016). Helicobacter pylori vacA transcription is genetically-determined and stratifies the level of human gastric inflammation and atrophy. Journal of Clinical Pathology, 69(11), 968-973. https://doi.org/10.1136/jclinpath-2016-203641
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 7, 2016 |
Online Publication Date | May 17, 2016 |
Publication Date | Nov 30, 2016 |
Deposit Date | Apr 20, 2016 |
Publicly Available Date | Mar 29, 2024 |
Journal | Journal of Clinical Pathology |
Print ISSN | 0021-9746 |
Electronic ISSN | 1472-4146 |
Publisher | BMJ Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 69 |
Issue | 11 |
Pages | 968-973 |
DOI | https://doi.org/10.1136/jclinpath-2016-203641 |
Keywords | Toxin; Microbial; Pathogenic; Helicobacter pylori; Inflammation, Gastric pathology |
Public URL | https://nottingham-repository.worktribe.com/output/786171 |
Publisher URL | https://jcp.bmj.com/content/69/11/968 |
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Helicobacter pylori vacA transcription is geneticallydetermined and stratifies the level of human gastric inflammation and atrophy
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https://creativecommons.org/licenses/by/4.0/
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