Skip to main content

Research Repository

Advanced Search

Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants: Digital phenotyping of root system architecture

Piñeros, Miguel A.; Larson, Brandon G.; Shaff, Jon E.; Schneider, David J.; Falcão, Alexandre Xavier; Yuan, Lixing; Clark, Randy T.; Craft, Eric J.; Davis, Tyler W.; Pradier, Pierre-Luc; Shaw, Nathanael M.; Assaranurak, Ithipong; McCouch, Susan R.; Sturrock, Craig; Bennett, Malcolm J.; Kochian, Leon V.

Authors

Miguel A. Piñeros

Brandon G. Larson

Jon E. Shaff

David J. Schneider

Alexandre Xavier Falcão

Lixing Yuan

Randy T. Clark

Eric J. Craft

Tyler W. Davis

Pierre-Luc Pradier

Nathanael M. Shaw

Ithipong Assaranurak

Susan R. McCouch

Leon V. Kochian



Abstract

A plant's ability to maintain or improve its yield under limiting conditions, such as nutrient deficiency or drought, can be strongly influenced by root system architecture (RSA), the three-dimensional distribution of the different root types in the soil. The ability to image, track and quantify these root system attributes in a dynamic fashion is a useful tool in assessing desirable genetic and physiological root traits. Recent advances in imaging technology and phenotyping software have resulted in substantive progress in describing and quantifying RSA. We have designed a hydroponic growth system which retains the three-dimensional RSA of the plant root system, while allowing for aeration, solution replenishment and the imposition of nutrient treatments, as well as high-quality imaging of the root system. The simplicity and flexibility of the system allows for modifications tailored to the RSA of different crop species and improved throughput. This paper details the recent improvements and innovations in our root growth and imaging system which allows for greater image sensitivity (detection of fine roots and other root details), higher efficiency, and a broad array of growing conditions for plants that more closely mimic those found under field conditions.

Citation

Piñeros, M. A., Larson, B. G., Shaff, J. E., Schneider, D. J., Falcão, A. X., Yuan, L., …Kochian, L. V. (2016). Evolving technologies for growing, imaging and analyzing 3D root system architecture of crop plants: Digital phenotyping of root system architecture. Journal of Integrative Plant Biology, 58(3), 230-241. https://doi.org/10.1111/jipb.12456

Journal Article Type Article
Acceptance Date Dec 16, 2015
Online Publication Date Mar 17, 2016
Publication Date 2016-03
Deposit Date Aug 4, 2016
Publicly Available Date Aug 4, 2016
Journal Journal of Integrative Plant Biology
Print ISSN 1672-9072
Electronic ISSN 1672-9072
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 58
Issue 3
Pages 230-241
DOI https://doi.org/10.1111/jipb.12456
Keywords Abiotic stress; digital root phenotyping; mineral nutrition;
root system architecture
Public URL http://eprints.nottingham.ac.uk/id/eprint/35708
Publisher URL http://onlinelibrary.wiley.com/doi/10.1111/jipb.12456/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf

Files


Pineros et al Reprint - Root Architecture Phenotyping Improvements -JIPB 2016.pdf (5.3 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





You might also like



Downloadable Citations