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Formin Is Associated with Left-Right Asymmetry in the Pond Snail and the Frog

Davison, Angus; McDowell, Gary S.; Holden, Jennifer M.; Johnson, Harriet F.; Koutsovoulos, Georgios D.; Liu, M. Maureen; Hulpiau, Paco; Roy, Frans Van; Wade, Christopher M.; Banerjee, Ruby; Yang, Fengtang; Chiba, Satoshi; Davey, John W.; Jackson, Daniel J.; Levin, Michael; Blaxter, Mark L.

Authors

ANGUS DAVISON angus.davison@nottingham.ac.uk
Associate Professor and Reader in Evolutionary Genetics

Gary S. McDowell

Jennifer M. Holden

Harriet F. Johnson

Georgios D. Koutsovoulos

M. Maureen Liu

Paco Hulpiau

Frans Van Roy

CHRIS WADE chris.wade@nottingham.ac.uk
Assistant Professor

Ruby Banerjee

Fengtang Yang

Satoshi Chiba

John W. Davey

Daniel J. Jackson

Michael Levin

Mark L. Blaxter



Abstract

While components of the pathway that establishes left-right asymmetry have been identified in diverse animals, from vertebrates to flies, it is striking that the genes involved in the first symmetry-breaking step remain wholly unknown in the most obviously chiral animals, the gastropod snails. Previously, research on snails was used to show that left-right signalling of Nodal, downstream of symmetry-breaking, may be an ancestral feature of the Bilateria. Here we report that a disabling mutation in one copy of a tandemly duplicated, diaphanous-related formin is perfectly associated with symmetry-breaking in the pond snail. This is supported by the observation that an anti-formin drug treatment converts dextral snail embryos to a sinistral phenocopy, and in frogs, drug inhibition or over-expression by microinjection of formin has a chirality-randomizing effect in early (pre-cilia) embryos. Contrary to expectations based on existing models, we discovered asymmetric gene expression in 2 and 4 cell snail embryos, preceding morphological asymmetry. As the formin-actin filament has been shown to be part of an asymmetry-breaking switch in vitro, together these results are consistent with the view that animals with diverse bodyplans may derive their asymmetries from the same intracellular chiral elements.

Journal Article Type Article
Publication Date Mar 7, 2016
Journal Current Biology
Print ISSN 0960-9822
Electronic ISSN 1879-0445
Publisher Elsevier (Cell Press)
Peer Reviewed Peer Reviewed
Volume 26
Issue 5
Pages 654-660
APA6 Citation Davison, A., McDowell, G. S., Holden, J. M., Johnson, H. F., Koutsovoulos, G. D., Liu, M. M., …Blaxter, M. L. (2016). Formin Is Associated with Left-Right Asymmetry in the Pond Snail and the Frog. Current Biology, 26(5), 654-660. https://doi.org/10.1016/j.cub.2015.12.071
DOI https://doi.org/10.1016/j.cub.2015.12.071
Publisher URL http://www.cell.com/current-biology/fulltext/S0960-9822(16)00056-7
Related Public URLs http://dx.doi.org/10.1016/j.cub.2015.12.071
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information This article is maintained by: Elsevier; Article Title: Formin Is Associated with Left-Right Asymmetry in the Pond Snail and the Frog; Journal Title: Current Biology; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.cub.2015.12.071; Content Type: article; Copyright: Copyright © 2016 The Authors. Published by Elsevier Ltd.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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