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Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle

Salmon, Andrew H. J.; Lobo, Daniela P.; Wemyss, Alan M.; Smith, David J.; Straube, Anne; Betteridge, Kai B.; Salmon, Andrew H.J.; Foster, Rebecca R.; Elhegni, Hesham E.; Satchell, Simon C.; Little, Haydn A.; Pacheco-Gómez, Raúl; Simmons, Mark J.; Hicks, Matthew R.; Bates, David O.; Rodger, Alison; Dafforn, Timothy R.; Arkill, Kenton P.

Authors

Andrew H. J. Salmon

Daniela P. Lobo

Alan M. Wemyss

David J. Smith

Anne Straube

Kai B. Betteridge

Andrew H.J. Salmon

Rebecca R. Foster

Hesham E. Elhegni

Simon C. Satchell

Haydn A. Little

Raúl Pacheco-Gómez

Mark J. Simmons

Matthew R. Hicks

Alison Rodger

Timothy R. Dafforn

Kenton P. Arkill



Abstract

The wall shear stress (WSS) that a moving fluid exerts on a surface affects many processes including those relating to vascular function. WSS plays an important role in normal physiology (e.g. angiogenesis) and affects the microvasculature's primary function of molecular transport. Points of fluctuating WSS show abnormalities in a number of diseases; however, there is no established technique for measuring WSS directly in physiological systems. All current methods rely on estimates obtained from measured velocity gradients in bulk flow data. In this work, we report a nanosensor that can directly measure WSS in microfluidic chambers with sub-micron spatial resolution by using a specific type of virus, the bacteriophage M13, which has been fluorescently labeled and anchored to a surface. It is demonstrated that the nanosensor can be calibrated and adapted for biological tissue, revealing WSS in micro-domains of cells that cannot be calculated accurately from bulk flow measurements. This method lends itself to a platform applicable to many applications in biology and microfluidics.

Journal Article Type Article
Publication Date 2015-10
Journal Nano Research
Print ISSN 1998-0124
Electronic ISSN 1998-0000
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 8
Issue 10
Pages 3307-3315
APA6 Citation Salmon, A. H. J., Lobo, D. P., Wemyss, A. M., Smith, D. J., Straube, A., Betteridge, K. B., …Arkill, K. P. (2015). Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle. Nano Research, 8(10), 3307-3315. https://doi.org/10.1007/s12274-015-0831-x
DOI https://doi.org/10.1007/s12274-015-0831-x
Publisher URL https://link.springer.com/article/10.1007%2Fs12274-015-0831-x
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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