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Investigating lung responses with functional hyperpolarized xenon-129 MRI in an ex vivo rat model of asthma: Hyperpolarized129Xe MRI in OVA Induced Asthma

Lilburn, David M.L.; Tatler, Amanda L.; Six, Joseph S.; Lesbats, Clémentine; Habgood, Antony; Porte, Joanne; Hughes-Riley, Theodore; Shaw, Dominick E.; Jenkins, Gisli; Meersmann, Thomas

Authors

David M.L. Lilburn

Joseph S. Six

Clémentine Lesbats

Antony Habgood

Joanne Porte

Theodore Hughes-Riley

GISLI JENKINS gisli.jenkins@nottingham.ac.uk
Professor of Experimental Medicine



Abstract

Purpose: Asthma is a disease of increasing worldwide importance that calls for new investigative methods. Ex vivo lung tissue is being increasingly used to study functional respiratory parameters independent of confounding systemic considerations but also to reduce animal numbers and associated research costs. In this work a straightforward laboratory method is advanced to probe dynamic changes in gas inhalation patterns by utilizing an ex vivo small animal ovalbumin (OVA) model of human asthma.
Methods: Hyperpolarized (hp) 129Xe was actively inhaled by the excised lungs exposed to a constant pressure differential that mimicked negative pleural cavity pressure. The method enabled hp 129Xe MRI of airway responsiveness to intravenous methacholine (MCh) and airway challenge reversal through salbutamol.
Results: Significant differences were demonstrated between control and OVA challenged animals on global lung hp 129Xe gas inhalation with p < 0.05 at MCh dosages above 460 μg. Spatial mapping of the regional hp gas distribution revealed an approximately 3 fold increase in heterogeneity for the asthma model organs.
Conclusion: The experimental results from this proof of concept work suggest that the ex vivo hp noble gas imaging arrangement and the applied image analysis methodology may be useful as an adjunct to current diagnostic techniques.

Journal Article Type Article
Publication Date Oct 28, 2015
Journal Magnetic Resonance in Medicine
Print ISSN 0740-3194
Electronic ISSN 1522-2594
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 76
Issue 4
Pages 1224-1235
APA6 Citation Habgood, A., Lilburn, D. M., Tatler, A. L., Six, J. S., Lesbats, C., Habgood, A., …Meersmann, T. (2015). Investigating lung responses with functional hyperpolarized xenon-129 MRI in an ex vivo rat model of asthma: Hyperpolarized129Xe MRI in OVA Induced Asthma. Magnetic Resonance in Medicine, 76(4), 1224-1235. https://doi.org/10.1002/mrm.26003
DOI https://doi.org/10.1002/mrm.26003
Keywords pulmonary imaging; hyperpolarized noble gas
MRI; hp 129Xe; ovalbumin (OVA) rat model of asthma; methacholine challenges; airway hyper-responsiveness
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/mrm.26003/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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