Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates

Fornari, Enzo; Roberts, Clive J.; Temperton, Robert H.; O�Shea, James N.

Abstract

We demonstrate in this work the deposition of a large biological molecule (fibronectin) on polymeric substrates in a high vacuum environment using an electrospray deposition system. Fibronectin was deposited and its distribution and structure investigated and retention of function (ability to promote cell adhesion) on return to liquid environment is shown. AFM was used to monitor changes in the morphology of the surface before and after fibronectin deposition, whilst the biological activity of the deposited protein is assessed through a quantitative analysis of the biomolecular adhesion and migration of fibroblast cells to the modified surfaces. For the first time we have demonstrated that using high vacuum electrospray deposition it is possible to deposit large protein molecules on polymeric surfaces whilst maintaining the protein activity. The deposition of biological molecules such as proteins with the retention of their activity onto clean well-controlled surfaces under vacuum condition, offers the possibility for future studies utilizing high resolution vacuum based techniques at the atomic and molecular scale providing a greater understanding of protein–surface interface behaviour of relevance to a wide range of applications such as in sensors, diagnostics and tissue engineering.

Citation

Fornari, E., Roberts, C. J., Temperton, R. H., & O’Shea, J. N. (2015). Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates. Journal of Colloid and Interface Science, 453, 252-259. https://doi.org/10.1016/j.jcis.2015.05.007

• Journal Article Type: Article
• Acceptance Date: May 5, 2015
• Online Publication Date: May 13, 2015
• Publication Date: 2015-09
• Deposit Date: Jul 26, 2016
• Publicly Available Date: Jul 26, 2016
• Journal: Journal of Colloid and Interface Science
• Print ISSN: 0021-9797
• Electronic ISSN: 1095-7103
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 453
• Pages: 252-259
• DOI: https://doi.org/10.1016/j.jcis.2015.05.007
• Keywords: Vacuum electrospray deposition; Fibronectin deposition; Cell adhesion and migration; Protein activity
• Public URL: https://nottingham-repository.worktribe.com/output/757964
• Publisher URL: http://www.sciencedirect.com/science/article/pii/S0021979715004610
• Additional Information: This article is maintained by: Elsevier; Article Title: Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates; Journal Title: Journal of Colloid and Interface Science; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jcis.2015.05.007; Content Type: article; Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.