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Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates

Fornari, Enzo; Roberts, Clive J.; Temperton, Robert H.; O’Shea, James N.

Authors

Enzo Fornari enzofornari@hotmail.co.uk

Clive J. Roberts

Robert H. Temperton

James N. O’Shea



Abstract

We demonstrate in this work the deposition of a large biological molecule (fibronectin) on polymeric substrates in a high vacuum environment using an electrospray deposition system. Fibronectin was deposited and its distribution and structure investigated and retention of function (ability to promote cell adhesion) on return to liquid environment is shown. AFM was used to monitor changes in the morphology of the surface before and after fibronectin deposition, whilst the biological activity of the deposited protein is assessed through a quantitative analysis of the biomolecular adhesion and migration of fibroblast cells to the modified surfaces. For the first time we have demonstrated that using high vacuum electrospray deposition it is possible to deposit large protein molecules on polymeric surfaces whilst maintaining the protein activity. The deposition of biological molecules such as proteins with the retention of their activity onto clean well-controlled surfaces under vacuum condition, offers the possibility for future studies utilizing high resolution vacuum based techniques at the atomic and molecular scale providing a greater understanding of protein–surface interface behaviour of relevance to a wide range of applications such as in sensors, diagnostics and tissue engineering.

Journal Article Type Article
Publication Date 2015-09
Journal Journal of Colloid and Interface Science
Print ISSN 0021-9797
Electronic ISSN 1095-7103
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 453
Pages 252-259
APA6 Citation Fornari, E., Roberts, C. J., Temperton, R. H., & O’Shea, J. N. (2015). Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates. Journal of Colloid and Interface Science, 453, 252-259. https://doi.org/10.1016/j.jcis.2015.05.007
DOI https://doi.org/10.1016/j.jcis.2015.05.007
Keywords Vacuum electrospray deposition; Fibronectin deposition; Cell adhesion and migration; Protein activity
Publisher URL http://www.sciencedirect.com/science/article/pii/S0021979715004610
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0
Additional Information This article is maintained by: Elsevier; Article Title: Electrospray deposition in vacuum as method to create functionally active protein immobilization on polymeric substrates; Journal Title: Journal of Colloid and Interface Science; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.jcis.2015.05.007; Content Type: article; Copyright: Copyright © 2015 Elsevier Inc. All rights reserved.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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