John R. Glossop
DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS
Glossop, John R.; Haworth, Kim E.; Emes, Richard D.; Nixon, Nicola B.; Packham, Jon C.; Dawes, Peter T.; Fryer, Anthony A.; Mattey, Derek L.; Farrell, William E.
Kim E. Haworth
Richard D. Emes
Nicola B. Nixon
Jon C. Packham
Peter T. Dawes
Anthony A. Fryer
Derek L. Mattey
William E. Farrell
Aims: Alterations in DNA methylation contribute to the abnormal phenotype of fibroblast-like synoviocytes (FLS) from patients with rheumatoid arthritis (RA). Herein, we performed genome-wide DNA methylation profiling of RA FLS derived from synovial fluid, a more readily accessible source of disease-associated cells.
Patients & Methods: Synovial fluid-derived FLS were isolated and expanded in vitro from 12 patients with RA and osteoarthritis (OA). DNA methylation was interrogated using HumanMethylation450 BeadChips and bisulfite pyrosequencing.
Results: Array analysis identified 328 CpGs, representing 195 genes, that were differentially methylated between RA and OA fluid-derived FLS. Comparison with the genes identified in two independent studies of tissue-derived FLS revealed 73 genes in common (~40%), of which 22 shared identity with both studies. Pyrosequencing confirmed altered methylation of these genes.
Conclusions: Synovial fluid-derived RA FLS show methylation changes across multiple genes. A subset of these are common with tissue-derived FLS, supporting the use of fluid-derived FLS for future investigations.
|Journal Article Type||Article|
|Publication Date||Jun 1, 2015|
|Peer Reviewed||Peer Reviewed|
|APA6 Citation||Glossop, J. R., Haworth, K. E., Emes, R. D., Nixon, N. B., Packham, J. C., Dawes, P. T., …Farrell, W. E. (2015). DNA methylation profiling of synovial fluid FLS in rheumatoid arthritis reveals changes common with tissue-derived FLS. Epigenomics, 7(4), https://doi.org/10.2217/epi.15.15|
|Keywords||Rheumatoid arthritis; DNA Methylation; Fibroblast-like synoviocyte; Synovial fluid; Epigenetics; Osteoarthritis|
|Copyright Statement||Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf|
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf
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