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ELISA in the multiplex era: potentials and pitfalls

Tighe, Patrick J.; Ryder, Richard R.; Todd, Ian; Fairclough, Lucy C.

Authors

Richard R. Ryder

Ian Todd

Lucy C. Fairclough



Abstract

Multiplex immunoassays confer several advantages over widely adopted singleplex immunoassays including increased efficiency at a reduced expense, greater output per sample volume ratios and higher throughput predicating more resolute, detailed diagnostics and facilitating personalised medicine. Nonetheless, to date, relatively few protein multiplex immunoassays have been validated for in vitro diagnostics in clinical/point-of-care settings. This review article will outline the challenges, which must be ameliorated prior to the widespread integration of multiplex immunoassays in clinical settings: (i) biomarker validation; (ii) standardisation of immunoassay design and quality control (calibration and quantification); (iii) availability, stability, specificity and cross-reactivity of reagents; (iv) assay automation and the use of validated algorithms for transformation of raw data into diagnostic results. A compendium of multiplex immunoassays applicable to in vitro diagnostics and a summary of the diagnostic products currently available commercially are included, along with an analysis of the relative states of development for each format (namely planar slide based, suspension and planar/microtitre plate based) with respect to the aforementioned issues.

Citation

Tighe, P. J., Ryder, R. R., Todd, I., & Fairclough, L. C. (2015). ELISA in the multiplex era: potentials and pitfalls. PROTEOMICS - Clinical Applications, 9(3-4), https://doi.org/10.1002/prca.201400130

Journal Article Type Article
Acceptance Date Jan 19, 2015
Online Publication Date Mar 25, 2015
Publication Date Apr 20, 2015
Deposit Date Feb 22, 2017
Publicly Available Date Feb 22, 2017
Journal Proteomics Clinical Applications
Print ISSN 1862-8346
Electronic ISSN 1862-8354
Publisher Wiley-VCH Verlag
Peer Reviewed Peer Reviewed
Volume 9
Issue 3-4
DOI https://doi.org/10.1002/prca.201400130
Keywords ELISA, In vitro diagnostics, Multiplex immunoassay
Public URL http://eprints.nottingham.ac.uk/id/eprint/40706
Publisher URL http://onlinelibrary.wiley.com/doi/10.1002/prca.201400130/abstract
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0





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