ELISA in the multiplex era: potentials and pitfalls

Tighe, Patrick J.; Ryder, Richard R.; Todd, Ian; Fairclough, Lucy C.

Abstract

Multiplex immunoassays confer several advantages over widely adopted singleplex immunoassays including increased efficiency at a reduced expense, greater output per sample volume ratios and higher throughput predicating more resolute, detailed diagnostics and facilitating personalised medicine. Nonetheless, to date, relatively few protein multiplex immunoassays have been validated for in vitro diagnostics in clinical/point-of-care settings. This review article will outline the challenges, which must be ameliorated prior to the widespread integration of multiplex immunoassays in clinical settings: (i) biomarker validation; (ii) standardisation of immunoassay design and quality control (calibration and quantification); (iii) availability, stability, specificity and cross-reactivity of reagents; (iv) assay automation and the use of validated algorithms for transformation of raw data into diagnostic results. A compendium of multiplex immunoassays applicable to in vitro diagnostics and a summary of the diagnostic products currently available commercially are included, along with an analysis of the relative states of development for each format (namely planar slide based, suspension and planar/microtitre plate based) with respect to the aforementioned issues.

Citation

Tighe, P. J., Ryder, R. R., Todd, I., & Fairclough, L. C. (2015). ELISA in the multiplex era: potentials and pitfalls. PROTEOMICS - Clinical Applications, 9(3-4), https://doi.org/10.1002/prca.201400130

• Journal Article Type: Article
• Acceptance Date: Jan 19, 2015
• Online Publication Date: Mar 25, 2015
• Publication Date: Apr 20, 2015
• Deposit Date: Feb 22, 2017
• Publicly Available Date: Feb 22, 2017
• Journal: Proteomics Clinical Applications
• Print ISSN: 1862-8346
• Electronic ISSN: 1862-8354
• Publisher: Wiley-VCH Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 9
• Issue: 3-4
• DOI: https://doi.org/10.1002/prca.201400130
• Keywords: ELISA, In vitro diagnostics, Multiplex immunoassay
• Public URL: https://nottingham-repository.worktribe.com/output/749465
• Publisher URL: http://onlinelibrary.wiley.com/doi/10.1002/prca.201400130/abstract
• Contract Date: Feb 22, 2017