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Insight into proteomic investigations of Neisseria meningitidis serogroup C strain L91543 from analysis of its genome sequence

Karlyshev, A.V.; Snyder, Lori A.S.; McFadden, Johnjoe; Griffin, R.

Authors

A.V. Karlyshev a.karlyshev@kingston.ac.uk

Lori A.S. Snyder l.snyder@kingston.ac.uk

Johnjoe McFadden j.mcfadden@surrey.ac.uk

R. Griffin



Abstract

Here, we describe the draft sequence of a virulent isolate of Neisseria meningitidis strain L91543, belonging to serogroup C. The findings from previous proteomic and metabolomic studies of this strain can now be further interpreted with genomic analysis. Comparative analysis of the genome sequence revealed close similarity and localized synteny with the genome sequence of N. meningitidis serogroup C strain, FAM18. Polymorphisms were identified in the signal peptide sequence of factor H binding protein, a target for new meningococcal vaccines, which may result in its inefficient translocation across the cytoplasmic membrane affecting its processing (lipidation and cleavage of the signal peptide) and transportation to the outer membrane in strain L91543. This would explain the unusual proteomic data for factor H binding protein for this strain. NadA, another target for new vaccines, and the MtrR regulator, which controls expression of NadA, both contain SNPs between strains L91543 and FAM18. The genome sequence data were generated using Ion Torrent PGM sequencing, assembled into 50 contigs with 64× coverage and annotated with 2262 genes, 14 rRNAs and 56 tRNAs. The availability of the genome of N. meningitidis strain L91543 will aid our understanding of the proteome of this organism, importantly its vaccine antigens.

Journal Article Type Article
Publication Date May 1, 2015
Journal FEMS Microbiology Letters
Print ISSN 0378-1097
Electronic ISSN 1574-6968
Publisher Oxford University Press (OUP)
Peer Reviewed Peer Reviewed
Volume 362
Issue 9
Article Number fnv055
APA6 Citation Karlyshev, A., Snyder, L. A., McFadden, J., & Griffin, R. (2015). Insight into proteomic investigations of Neisseria meningitidis serogroup C strain L91543 from analysis of its genome sequence. FEMS Microbiology Letters, 362(9), doi:10.1093/femsle/fnv055
DOI https://doi.org/10.1093/femsle/fnv055
Keywords Meningococcus; Next-generation genome sequencing; Ion Torrent PGM; Meningitis; Comparative genomics
Publisher URL https://academic.oup.com/femsle/article/362/9/fnv055/549247
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This article has also been deposited in the repository of Kingston University at http://eprints.kingston.ac.uk/31335/
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