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Nuclear transfer in ruminants

Lee, Joon Hee; Maalouf, Walid E.


Joon Hee Lee

Walid E. Maalouf


Nathalie Beaujean

Hélène Jammes

Alice Jouneau


© Springer Science+Business Media New York 2015. Ruminants were the first mammalian species to be cloned successfully by nuclear transplantation. Those experiments were designed to multiply high merit animals (Willadsen, Nature 320(6057):63–65, 1986; Prather et al., Biol Reprod 37(4):859–866, 1987; Wilmut et al., Nature 385(6619):810–813, 1997). Since then, cloning has provided us with a vast amount of knowledge and information on the reprogramming ability of somatic cells to different cell types which became an important basis for stem cell research and human medicine. Nowadays, the goals of most nuclear transfer work vary widely but in most cases the micromanipulation procedures remain the same. However, differences between species require different technical considerations. In this chapter, we describe in detail somatic cell nuclear transfer which is the foremost method for cloning ruminants with specific reference to sheep and cattle.


Lee, J. H., & Maalouf, W. E. (2014). Nuclear transfer in ruminants. In H. Jammes, N. Beaujean, & A. Jouneau (Eds.), Nuclear reprogramming: methods and protocols, (25-36). Springer New York.

Acceptance Date Jul 26, 2014
Online Publication Date Sep 20, 2014
Publication Date Sep 20, 2014
Deposit Date Feb 7, 2017
Publicly Available Date Feb 7, 2017
Electronic ISSN 1064-3745
Peer Reviewed Peer Reviewed
Issue 1222
Pages 25-36
Series Title Methods in molecular biology
Series Number 1222
Book Title Nuclear reprogramming: methods and protocols
ISBN 9781493915934; 9781493915941
Public URL
Publisher URL
Copyright Statement Copyright information regarding this work can be found at the following address:


Lee_and_Maalouf-2014-MethodsMolecularBiology submitted.pdf (309 Kb)

Copyright Statement
Copyright information regarding this work can be found at the following address:

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