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Nuclear transfer in ruminants

Lee, Joon Hee; Maalouf, Walid E.

Authors

Joon Hee Lee



Contributors

Nathalie Beaujean
Editor

Hélène Jammes
Editor

Alice Jouneau
Editor

Abstract

© Springer Science+Business Media New York 2015. Ruminants were the first mammalian species to be cloned successfully by nuclear transplantation. Those experiments were designed to multiply high merit animals (Willadsen, Nature 320(6057):63–65, 1986; Prather et al., Biol Reprod 37(4):859–866, 1987; Wilmut et al., Nature 385(6619):810–813, 1997). Since then, cloning has provided us with a vast amount of knowledge and information on the reprogramming ability of somatic cells to different cell types which became an important basis for stem cell research and human medicine. Nowadays, the goals of most nuclear transfer work vary widely but in most cases the micromanipulation procedures remain the same. However, differences between species require different technical considerations. In this chapter, we describe in detail somatic cell nuclear transfer which is the foremost method for cloning ruminants with specific reference to sheep and cattle.

Publication Date Sep 20, 2014
Electronic ISSN 1064-3745
Peer Reviewed Peer Reviewed
Issue 1222
Pages 25-36
Series Title Methods in molecular biology
Series Number 1222
Book Title Nuclear reprogramming: methods and protocols
ISBN 9781493915934; 9781493915941
APA6 Citation Lee, J. H., & Maalouf, W. E. (2014). Nuclear transfer in ruminants. In H. Jammes, N. Beaujean, & A. Jouneau (Eds.), Nuclear reprogramming: methods and protocols, (25-36). Springer New York. https://doi.org/10.1007/978-1-4939-1594-1_3
DOI https://doi.org/10.1007/978-1-4939-1594-1_3
Publisher URL http://dx.doi.org/10.1007/978-1-4939-1594-1_3
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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