Alessandro Sinopoli
The role of copper(ii) in the aggregation of human amylin
Sinopoli, Alessandro; Magr�, Antonio; Milardi, Danilo; Pappalardo, Matteo; Pucci, Pietro; Flagiello, Angela; Titman, Jeremy J.; Nicoletti, Vincenzo G.; Caruso, Giuseppe; Pappalardo, Giuseppe; Grasso, Giuseppe
Authors
Antonio Magr�
Danilo Milardi
Matteo Pappalardo
Pietro Pucci
Angela Flagiello
Jeremy J. Titman
Vincenzo G. Nicoletti
Giuseppe Caruso
Giuseppe Pappalardo
Giuseppe Grasso
Abstract
Amylin is the 37-residue peptide hormone produced by the islet ?-cells in the pancreas and the formation of amylin aggregates is strongly associated with ?-cells degeneration in type 2 diabetes, as demonstrated by more than 95% of patients exhibiting amylin amyloid upon autopsy. It is widely recognized that metal ions such as copper(II) have been implicated in the aggregation process of amyloidogenic peptides such as A? and ?-synuclein and there is evidence that also amylin self-assembly is largely affected by copper(II). For this reason, in this work, the role of copper(II) in the aggregation of amylin has been investigated by several different experimental approaches. Mass spectrometric investigations show that copper(II) induces significant changes in the amylin structure which decrease the protein fibrillogenesis as observed by ThT measurements. Accordingly, solid-state NMR experiments together with computational analysis carried out on a model amylin fragment confirmed the non fibrillogenic nature of the copper(II) induced aggregated structure. Finally, the presence of copper(II) is also shown to have a major influence on amylin proneness to be degraded by proteases and cytotoxicity studies on different cell cultures are reported.
Citation
Sinopoli, A., Magrì, A., Milardi, D., Pappalardo, M., Pucci, P., Flagiello, A., …Grasso, G. (2014). The role of copper(ii) in the aggregation of human amylin. Metallomics, 6(10), 1841-1852. https://doi.org/10.1039/c4mt00130c
Journal Article Type | Article |
---|---|
Acceptance Date | Jul 24, 2014 |
Publication Date | Oct 1, 2014 |
Deposit Date | Aug 18, 2014 |
Publicly Available Date | Oct 1, 2014 |
Journal | Metallomics |
Print ISSN | 1756-5901 |
Electronic ISSN | 1756-591X |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 6 |
Issue | 10 |
Pages | 1841-1852 |
DOI | https://doi.org/10.1039/c4mt00130c |
Public URL | https://nottingham-repository.worktribe.com/output/734849 |
Publisher URL | http://pubs.rsc.org/en/content/articlelanding/2014/mt/c4mt00130c#!divAbstract |
Additional Information | : This document is CrossCheck deposited; : Supplementary Information; : The Royal Society of Chemistry has an exclusive publication licence for this journal; OPEN ACCESS: The accepted version of this article will be made freely available after a 12 month embargo period; : Received 6 May 2014; Accepted 24 July 2014; Accepted Manuscript published 24 July 2014; Advance Article published 1 August 2014; Version of Record published 1 October 2014 |
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