Methods for assessing the regenerative responses of neural tissue

Poser, Steven W.; Rueger, Maria Adele; Androutsellis-Theotokis, Andreas

Abstract

In order to establish novel therapeutic paradigms and advance the field of regenerative medicine, methods for their effective implementation as well as rigorous assessment of outcomes are critical. This is especially evident and challenging in the context of treating complex and devastating neurodegenerative disorders, such as Parkinson’s disease, multiple sclerosis, and ischemic stroke. Stem cell-based approaches offer great promise in addressing these conditions. Here, we demonstrate an approach for identifying factors that mobilize endogenous neural stem cells in the repair and recovery of the central nervous system of rodents, involving site-specific administration of growth factors that activate particular signal transduction pathways, and that allows for the assessment of outcome utilizing magnetic resonance imaging and immunohistochemistry.

Citation

Poser, S. W., Rueger, M. A., & Androutsellis-Theotokis, A. (2014). Methods for assessing the regenerative responses of neural tissue. In B. Christ, J. Oerlecke, & P. Stock (Eds.), Animal models for stem cell therapy (293-302). Humana Press. https://doi.org/10.1007/978-1-4939-1453-1_24

• Acceptance Date: Aug 4, 2014
• Publication Date: Aug 4, 2014
• Deposit Date: May 12, 2017
• Electronic ISSN: 1064-3745
• Publisher: Humana Press
• Peer Reviewed: Peer Reviewed
• Volume: 1213
• Issue: 1213
• Pages: 293-302
• Series Title: Methods in molecular biology
• Book Title: Animal models for stem cell therapy
• ISBN: 9781493914531
• DOI: https://doi.org/10.1007/978-1-4939-1453-1_24
• Keywords: Stem cells; Regenerative medicine; Neurodegeneration; Magnetic resonance imaging; Immunohistochemistry
• Public URL: https://nottingham-repository.worktribe.com/output/734508
• Publisher URL: https://doi.org/10.1007/978-1-4939-1453-1_24
• Contract Date: May 12, 2017