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Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka

Gajapathy, Kanapathy; Jude, Pavilupillai J.; Goodacre, S.L.; Peiris, Lalanthika B.S.; Ramasamy, Ranjan; Surendran, Sinnathamby N.

Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka Thumbnail


Authors

Kanapathy Gajapathy

Pavilupillai J. Jude

SARA GOODACRE sara.goodacre@nottingham.ac.uk
Professor of Evolutionary Biology and Genetics

Lalanthika B.S. Peiris

Ranjan Ramasamy

Sinnathamby N. Surendran



Abstract

Background: Anopheles barbirostris is a vector of malaria in Sri Lanka. The taxon exists as a species complex in the Southeast Asian region. Previous studies using molecular markers suggest that there are more than 4 distinct clades within the An. barbirostris complex in Southeast Asia. The present study characterizes Sri Lankan An. barbirostris using mtDNA cytochrome oxidase subunit I (COI) and ribosomal RNA internal transcribed spacer 2 (ITS2) gene sequences.

Findings: DNA was extracted from morphologically identified An. barbirostris specimens from Sri Lanka, the COI and ITS2 regions amplified and their sequences analysed by comparison with other GenBank entries. Maximum likelihood trees suggested that Sri Lankan An. barbirostris constitute a different molecular type most closely related to clade I.

Conclusions: Considering the uncorrected p distances between the clade I and Sri Lankan specimens it is fair to assume that the specimens collected from widely separated locations in Sri Lanka with morphology characteristic of An. barbirostris s.l. form a new molecular type with close resemblance to An. barbirostris s.s from Indonesia and Thailand.

Citation

Gajapathy, K., Jude, P. J., Goodacre, S., Peiris, L. B., Ramasamy, R., & Surendran, S. N. (2014). Molecular characterization of the malaria vector Anopheles barbirostris van der Wulp in Sri Lanka. Parasites and Vectors, 7(1), Article 348. https://doi.org/10.1186/1756-3305-7-348

Journal Article Type Article
Acceptance Date Jul 2, 2014
Publication Date Jul 29, 2014
Deposit Date Jul 26, 2016
Publicly Available Date Jul 26, 2016
Journal Parasites and Vectors
Electronic ISSN 1756-3305
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 7
Issue 1
Article Number 348
DOI https://doi.org/10.1186/1756-3305-7-348
Keywords Anopheles barbirostris, Malaria vector, Species complex, Sri Lanka
Public URL https://nottingham-repository.worktribe.com/output/731833
Publisher URL http://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-7-348
Contract Date Jul 26, 2016

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