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Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites

Peckham, R.K.; Brill, R.; Foster, David S.; Bowen, A.L.; Leigh, James A.; Coffey, Tracey J.; Flynn, Robin J.

Authors

R.K. Peckham

R. Brill

David S. Foster

A.L. Bowen

James A. Leigh

Tracey J. Coffey

Robin J. Flynn



Abstract

IL-17 has emerged as a key player in the immune system, exhibiting roles in protection from infectious diseases and promoting inflammation in autoimmunity. Initially thought to be CD4 T-cell-derived, the sources of IL-17 are now known to be varied and belong to both the innate and adaptive arms of the immune system. Mechanisms for inducing IL-17 production in lymphoid cells are thought to rely on appropriate antigenic stimulation in the context of TGF-β1, IL-6 and/or IL-1β. Using culture protocols adapted from human studies, we have effectively induced both bovine CD4+ and WC1+ γδ T-cells to produce IL-17 termed Th17 and γδ17 cells, respectively. The negative regulatory effect of IFN-γ on mouse and human IL-17 production can be extended to the bovine model, as addition of IFN-γ decreases IL-17 production in both cell types. Furthermore we show that infection with the protozoan Neospora caninum will induce fibroblasts to secrete pro-IL-17 factors thereby inducing a γδ17 phenotype that preferentially kills infected target cells. Our study identifies two T-cell sources of IL-17, and is the first to demonstrate a protective effect of IL-17+ T-cells in ruminants. Our findings offer further opportunities for future adjuvants or vaccines which could benefit from inducing these responses.

Citation

Peckham, R., Brill, R., Foster, D. S., Bowen, A., Leigh, J. A., Coffey, T. J., & Flynn, R. J. (2014). Two distinct populations of Bovine IL-17+ T-cells can be induced and WC1+IL-17+γδ T-cells are effective killers of protozoan parasites. Scientific Reports, 4, https://doi.org/10.1038/srep05431

Journal Article Type Article
Acceptance Date Jun 5, 2014
Online Publication Date Jun 25, 2014
Publication Date Jun 25, 2014
Deposit Date Oct 31, 2016
Publicly Available Date Oct 31, 2016
Journal Scientific Reports
Print ISSN 2045-2322
Electronic ISSN 2045-2322
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 4
Article Number 5431
DOI https://doi.org/10.1038/srep05431
Public URL http://eprints.nottingham.ac.uk/id/eprint/38085
Publisher URL http://www.nature.com/articles/srep05431
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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