Skip to main content

Research Repository

Advanced Search

Pigs naturally exposed to porcine circovirus type 2 (PCV2) generate antibody responses capable to neutralise PCV2 isolates of different genotypes and geographic origins

Kurtz, Sherry; Grau-Roma, Llorenc; Cortey, Marti; Fort, Maria; Rodríguez, Fernando; Sibila, Marina; Segalés, Joaquim

Authors

Sherry Kurtz

Llorenc Grau-Roma

Marti Cortey

Maria Fort

Fernando Rodríguez

Marina Sibila

Joaquim Segalés



Abstract

Porcine circovirus type 2 (PCV2) is the essential infectious agent for PCV2-systemic disease (PCV2-SD, formerly known as postweaning multisystemic wasting syndrome) and other pathological conditions. Recent studies indicated antigenic variability amongst different PCV2 isolates and suggested that single amino acid changes within the capsid protein determine differences in the level of neutralization by specific monoclonal antibodies. The objective of the present study was to examine the cross-reactivity of PCV2 antibodies induced in the context of a natural infection against different PCV2 isolates belonging to genotypes PCV2a and PCV2b. Sera taken from several farms from animals of varying health status (PCV2-SD and age-matched healthy pigs and a set of slaughter-aged animals) were assayed for neutralizing activity against four PCV2 isolates from both predominant genotypes (PCV2a and PCV2b) and of differing geographic origins (Europe and North-America). Results showed that most of studied pigs (79 out of 82) contained neutralizing antibodies (NA) able to neutralize all four studied viral strains. Overall, pigs had significantly higher NA titres against PCV2a than against PCV2b (P< 0.001). Accordingly, studied serums were able to better neutralize Burgos390L4 and Stoon-1010 strains (PCV2a) than L-33-Sp-10-54 and MO/S-06 strains (PCV2b) (P< 0.001). No differences between capabilities of seroneutralization of viruses from different geographic origin were observed. Present data suggests that sequence differences between PCV2 isolates translate to functional antigenic differences in viral neutralization in vivo.

Citation

Kurtz, S., Grau-Roma, L., Cortey, M., Fort, M., Rodríguez, F., Sibila, M., & Segalés, J. (2014). Pigs naturally exposed to porcine circovirus type 2 (PCV2) generate antibody responses capable to neutralise PCV2 isolates of different genotypes and geographic origins. Veterinary Research, 45, https://doi.org/10.1186/1297-9716-45-29

Journal Article Type Article
Acceptance Date Feb 21, 2014
Publication Date Mar 6, 2014
Deposit Date Oct 26, 2016
Publicly Available Date Oct 26, 2016
Journal Veterinary Research
Print ISSN 0928-4249
Electronic ISSN 1297-9716
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 45
Article Number 29
DOI https://doi.org/10.1186/1297-9716-45-29
Public URL http://eprints.nottingham.ac.uk/id/eprint/37934
Publisher URL http://dx.doi.org/10.1186/1297-9716-45-29
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

Kurtz et al., 2014.pdf (419 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations