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Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and T

Montowska, Magdalena; Rao, Wei; Alexander, Morgan R.; Tucker, Gregory A.; Barrett, David A.

Authors

Magdalena Montowska

Wei Rao

Morgan R. Alexander Morgan.Alexander@nottingham.ac.uk

Gregory A. Tucker

David A. Barrett



Abstract

The use of ambient desorption electrospray ionization (DESI-MS) mass spectrometry and liquid extraction surface analysis mass spectrometry (LESA-MS) is explored for the first time to analyse skeletal muscle proteins obtained from mixture of standard proteins and raw meat. Single proteins and mixtures of up to five proteins (myoglobin, troponin C, actin, BSA, tropomyosin) were deposited onto a polymer surface, followed by in-situ tryptic digestion and comparative analysis using DESI-MS and LESA-MS using tandem electrospray MS. Peptide peaks specific to individual proteins were readily distinguishable with good signal-to-noise ratio in the five-component mixture. LESA-MS gave a more stable analysis and greater sensitivity compared with DESI-MS. Meat tryptic digests were subjected to peptidomics analysis by DESI-MS and LESA-MS. Bovine, horse, pig, chicken and turkey muscle digests were clearly discriminated using multivariate data analysis (MVA) of the peptidomic datasets. The most abundant skeletal muscle proteins were identified and correctly classified according to the species following MS/MS analysis. The study shows, for the first time, that ambient ionization techniques such as DESI-MS and LESA-MS have great potential for species-specific analysis and differentiation of skeletal muscle proteins by direct surface desorption.

Journal Article Type Article
Publication Date May 6, 2014
Journal Analytical Chemistry
Print ISSN 0003-2700
Electronic ISSN 1520-6882
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 86
Issue 9
Pages 4479-4487
APA6 Citation Montowska, M., Rao, W., Alexander, M. R., Tucker, G. A., & Barrett, D. A. (2014). Tryptic Digestion Coupled with Ambient Desorption Electrospray Ionization and Liquid Extraction Surface Analysis Mass Spectrometry Enabling Identification of Skeletal Muscle Proteins in Mixtures and Distinguishing between Beef, Pork, Horse, Chicken, and Turkey Meat. Analytical Chemistry, 86(9), 4479-4487. https://doi.org/10.1021/ac5003432
DOI https://doi.org/10.1021/ac5003432
Keywords Desorption Electrospray Ionization-Mass Spectrometry, Liquid Extraction Surface Analysis-Mass Spectrometry, skeletal muscle proteins, cattle, pig, horse, chicken, turkey
Publisher URL http://pubs.acs.org/doi/10.1021/ac5003432
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf


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