A genome-wide metabolomic resource for tomato fruit from Solanum pennellii

Perez-Fons, Laura; Wells, Tom; Corol, Delia I.; Ward, Jane L.; Gerrish, Christopher; Beale, Michael H.; Seymour, Graham B.; Bramley, Peter M.; Fraser, Paul D.

Abstract

Tomato and its processed products are one of the most widely consumed fruits. Its domestication, however,
has resulted in the loss of some 95% of the genetic and chemical diversity of wild relatives. In order to
elucidate this diversity, exploit its potential for plant breeding, as well as understand its biological
significance, analytical approaches have been developed, alongside the production of genetic crosses of wild
relatives with commercial varieties. In this article, we describe a multi-platform metabolomic analysis, using
NMR, mass spectrometry and HPLC, of introgression lines ofSolanum pennellii with a domesticated line in
order to analyse and quantify alleles (QTL) responsible for metabolic traits. We have identified QTL for
health-related antioxidant carotenoids and tocopherols, as well as molecular signatures for some 2000
compounds. Correlation analyses have revealed intricate interactions in isoprenoid formation in the plastid
that can be extrapolated to other crop plants.

Citation

Perez-Fons, L., Wells, T., Corol, D. I., Ward, J. L., Gerrish, C., Beale, M. H., …Fraser, P. D. (in press). A genome-wide metabolomic resource for tomato fruit from Solanum pennellii. Scientific Reports, 4, Article 3859. https://doi.org/10.1038/srep03859

• Journal Article Type: Article
• Acceptance Date: Jan 2, 2014
• Online Publication Date: Jan 24, 2014
• Deposit Date: Feb 18, 2014
• Publicly Available Date: Feb 18, 2014
• Journal: Scientific Reports
• Electronic ISSN: 2045-2322
• Publisher: Nature Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 4
• Article Number: 3859
• DOI: https://doi.org/10.1038/srep03859
• Public URL: https://nottingham-repository.worktribe.com/output/721171
• Publisher URL: https://www.nature.com/articles/srep03859