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The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays

Tewari, Nilanjana; Zaitoun, Abed M.; Arora, Arvind; Madhusudan, Srinivasan; Ilyas, Mohammad; Lobo, Dileep N.

Authors

Nilanjana Tewari

Abed M. Zaitoun

Arvind Arora

Srinivasan Madhusudan

Mohammad Ilyas

Dileep N. Lobo

Abstract

Background
Tumour-associated lymphocytes (TALs) have been linked with good prognosis in several solid tumours. This study aimed to evaluate the prognostic significance of CD3, CD8 and CD20 positive lymphocytes in pancreatic ductal adenocarcinoma.

Methods
After histological re-evaluation of the tumours of 81 patients who underwent surgical resection for exclusively pancreatic ductal adenocarcinoma, tissue micro-arrays (TMA) were constructed and immunohistochemistry was performed for CD3, CD8 and CD20. The number of lymphocytes within specific tumour compartments (i.e. stromal and intratumoural) was quantified. X-tile software (Yale School of Medicine, CT, USA) was used to stratify patients into 'high’ and 'low’ for each of the lymphocytes stained and their association with survival. Receiver operating curves (ROC) were constructed to evaluate the association between the TALs, alone and in combination, with clinicopathological features.

Results
CD3 and CD8 positive lymphocytes were associated with grade of tumour differentiation. The presence of intratumoural CD3 positive cells was associated with improved survival (p = 0.028), and intratumoural and stromal CD3 in combination also correlated with improved survival (p = 0.043). When CD20 positive lymphocyte levels were high, survival improved (p = 0.029) and similar results were seen for CD20 in combination with intratumoural CD3 (p = 0.001) and stromal CD8 (p = 0.013).

Conclusions
This study has shown a correlation between the presence of TALs and survival in pancreatic ductal adenocarcinoma.

Journal Article Type Article
Publication Date Sep 24, 2013
Journal BMC Cancer
Electronic ISSN 1471-2407
Publisher Humana Press
Peer Reviewed Peer Reviewed
Volume 13
Article Number 436
Institution Citation Tewari, N., Zaitoun, A. M., Arora, A., Madhusudan, S., Ilyas, M., & Lobo, D. N. (2013). The presence of tumour-associated lymphocytes confers a good prognosis in pancreatic ductal adenocarcinoma: an immunohistochemical study of tissue microarrays. BMC Cancer, 13, doi:10.1186/1471-2407-13-436
DOI https://doi.org/10.1186/1471-2407-13-436
Keywords CD3; CD8; Pancreatic ductal adenocarcinoma; Tumour-associated lymphocytes; Prognosis; Immunohistochemistry
Publisher URL http://www.biomedcentral.com/1471-2407/13/436
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0




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