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Curcumin reduces prostaglandin E2, matrix metalloproteinase-3 and proteoglycan release in the secretome of interleukin 1?-treated articular cartilage

Clutterbuck, Abigail L.; Allaway, David; Harris, Pat; Mobasheri, Ali

Curcumin reduces prostaglandin E2, matrix metalloproteinase-3 and proteoglycan release in the secretome of interleukin 1?-treated articular cartilage Thumbnail


Authors

Abigail L. Clutterbuck

David Allaway

Pat Harris

Ali Mobasheri



Abstract

Objective: Curcumin (diferuloylmethane) is a phytochemical with potent anti-inflammatory and anti-oxidant properties, and has therapeutic potential for the treatment of a range of inflammatory diseases, including osteoarthritis (OA). The aim of this study was to determine whether non-toxic concentrations of curcumin can reduce interleukin-1beta (IL-1?)-stimulated inflammation and catabolism in an explant model of cartilage inflammation.
Methods: Articular cartilage explants and primary chondrocytes were obtained from equine metacarpophalangeal joints. Curcumin was added to monolayer cultured primary chondrocytes and cartilage explants in concentrations ranging from 3?M-100?M. Prostaglandin E2 (PGE2) and matrix metalloproteinase (MMP)-3 release into the secretome of IL-1?-stimulated explants was measured using a competitive ELISA and western blotting respectively. Proteoglycan (PG) release in the secretome was measured using the 1,9-dimethylmethylene blue (DMMB) assay. Cytotoxicity was assessed with a live/dead assay in monolayer cultures after 24 hours, 48 hours and five days, and in explants after five days.
Results: Curcumin induced chondrocyte death in primary cultures (50?M p

Journal Article Type Article
Publication Date Aug 20, 2013
Deposit Date Apr 10, 2014
Publicly Available Date Apr 10, 2014
Journal F1000Research
Electronic ISSN 2046-1402
Publisher F1000Research
Peer Reviewed Peer Reviewed
Volume 2
Article Number 147
DOI https://doi.org/10.12688/f1000research.2-147.v2
Public URL https://nottingham-repository.worktribe.com/output/716946
Publisher URL http://f1000research.com/articles/2-147/v2

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