Abigail L. Clutterbuck
Curcumin reduces prostaglandin E2, matrix metalloproteinase-3 and proteoglycan release in the secretome of interleukin 1?-treated articular cartilage
Clutterbuck, Abigail L.; Allaway, David; Harris, Pat; Mobasheri, Ali
Objective: Curcumin (diferuloylmethane) is a phytochemical with potent anti-inflammatory and anti-oxidant properties, and has therapeutic potential for the treatment of a range of inflammatory diseases, including osteoarthritis (OA). The aim of this study was to determine whether non-toxic concentrations of curcumin can reduce interleukin-1beta (IL-1?)-stimulated inflammation and catabolism in an explant model of cartilage inflammation.
Methods: Articular cartilage explants and primary chondrocytes were obtained from equine metacarpophalangeal joints. Curcumin was added to monolayer cultured primary chondrocytes and cartilage explants in concentrations ranging from 3?M-100?M. Prostaglandin E2 (PGE2) and matrix metalloproteinase (MMP)-3 release into the secretome of IL-1?-stimulated explants was measured using a competitive ELISA and western blotting respectively. Proteoglycan (PG) release in the secretome was measured using the 1,9-dimethylmethylene blue (DMMB) assay. Cytotoxicity was assessed with a live/dead assay in monolayer cultures after 24 hours, 48 hours and five days, and in explants after five days.
Results: Curcumin induced chondrocyte death in primary cultures (50?M p
Clutterbuck, A. L., Allaway, D., Harris, P., & Mobasheri, A. (2013). Curcumin reduces prostaglandin E2, matrix metalloproteinase-3 and proteoglycan release in the secretome of interleukin 1?-treated articular cartilage. F1000Research, 2, Article 147. https://doi.org/10.12688/f1000research.2-147.v2
|Journal Article Type||Article|
|Publication Date||Aug 20, 2013|
|Deposit Date||Apr 10, 2014|
|Publicly Available Date||Apr 10, 2014|
|Peer Reviewed||Peer Reviewed|
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0