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Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray

Fabi, João Paulo; Seymour, Graham B.; Graham, Neil S.; Broadley, Martin R.; May, Sean; Lajolo, Franco Maria; Cordenunsi, Beatriz Rosana; Oliveira do Nascimento, João Roberto

Authors

João Paulo Fabi

Graham B. Seymour graham.seymour@nottingham.ac.uk

Neil S. Graham

Martin R. Broadley

Sean May

Franco Maria Lajolo

Beatriz Rosana Cordenunsi

João Roberto Oliveira do Nascimento

Abstract

Background

Papaya (Carica papaya L.) is a commercially important crop that produces climacteric fruits with a soft and sweet pulp that contain a wide range of health promoting phytochemicals. Despite its importance, little is known about transcriptional modifications during papaya fruit ripening and their control. In this study we report the analysis of ripe papaya transcriptome by using a cross-species (XSpecies) microarray technique based on the phylogenetic proximity between papaya and Arabidopsis thaliana.

Results

Papaya transcriptome analyses resulted in the identification of 414 ripening-related genes with some having their expression validated by qPCR. The transcription profile was compared with that from ripening tomato and grape. There were many similarities between papaya and tomato especially with respect to the expression of genes encoding proteins involved in primary metabolism, regulation of transcription, biotic and abiotic stress and cell wall metabolism. XSpecies microarray data indicated that transcription factors (TFs) of the MADS-box, NAC and AP2/ERF gene families were involved in the control of papaya ripening and revealed that cell wall-related gene expression in papaya had similarities to the expression profiles seen in Arabidopsis during hypocotyl development.

Conclusion

The cross-species array experiment identified a ripening-related set of genes in papaya allowing the comparison of transcription control between papaya and other fruit bearing taxa during the ripening process.

Journal Article Type Article
Journal BMC Plant Biology
Electronic ISSN 1471-2229
Publisher Humana Press
Peer Reviewed Peer Reviewed
Volume 12
Article Number 242
Institution Citation Fabi, J. P., Seymour, G. B., Graham, N. S., Broadley, M. R., May, S., Lajolo, F. M., …Oliveira do Nascimento, J. R. (in press). Analysis of ripening-related gene expression in papaya using an Arabidopsis-based microarray. BMC Plant Biology, 12, doi:10.1186/1471-2229-12-242
DOI https://doi.org/10.1186/1471-2229-12-242
Keywords Oligo-chip, Heterologous microarray, Papaya ripening, Quantitative gene expression, Whole genome shotgun, Transcript profiling
Publisher URL http://dx.doi.org/10.1186/1471-2229-12-242
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0




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