Alasdair G. Kay
Therapeutic Effects of Hypoxic and Pro-Inflammatory Priming of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Inflammatory Arthritis
Kay, Alasdair G.; Treadwell, Kane; Roach, Paul; Morgan, Rebecca; Lodge, Rhys; Hyland, Mairead; Piccinini, Anna M.; Forsyth, Nicholas R.; Kehoe, Oksana
Authors
Kane Treadwell
Paul Roach
Rebecca Morgan
Rhys Lodge
Mairead Hyland
ANNA PICCININI ANNA.PICCININI@NOTTINGHAM.AC.UK
Assistant Professor
Nicholas R. Forsyth
Oksana Kehoe
Abstract
Mesenchymal stem cells (MSCs) immunomodulate inflammatory responses through paracrine signalling, including via secretion of extracellular vesicles (EVs) in the cell secretome. We evaluated the therapeutic potential of MSCs-derived small EVs in an antigen-induced model of arthritis (AIA). EVs isolated from MSCs cultured normoxically (21% O2, 5% CO2 ), hypoxically (2% O2, 5% CO2 ) or with a pro-inflammatory cytokine cocktail were applied into the AIA model. Disease pathology was assessed post-arthritis induction through swelling and histopathological analysis of synovial joint structure. Activated CD4+ T cells from healthy mice were cultured with EVs or MSCs to assess deactivation capabilities prior to application of standard EVs in vivo to assess T cell polarisation within the immune response to AIA. All EVs treatments reduced knee-joint swelling whilst only normoxic and pro-inflammatory primed EVs improved histopathological outcomes. In vitro culture with EVs did not achieve T cell deactivation. Polarisation towards CD4+ helper cells expressing IL17a (Th17) was reduced when normoxic and hypoxic EV treatments were applied in vitro. Normoxic EVs applied into the AIA model reduced Th17 polarisation and improved Regulatory T cell (Treg):Th17 homeostatic balance. Normoxic EVs present the optimal strategy for broad therapeutic benefit. EVs present an effective novel technology with the potential for cell-free therapeutic translation.
Citation
Kay, A. G., Treadwell, K., Roach, P., Morgan, R., Lodge, R., Hyland, M., …Kehoe, O. (2022). Therapeutic Effects of Hypoxic and Pro-Inflammatory Priming of Mesenchymal Stem Cell-Derived Extracellular Vesicles in Inflammatory Arthritis. International Journal of Molecular Sciences, 23(1), Article 126. https://doi.org/10.3390/ijms23010126
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 20, 2021 |
Online Publication Date | Dec 23, 2021 |
Publication Date | Jan 1, 2022 |
Deposit Date | Jan 4, 2022 |
Publicly Available Date | Jan 6, 2022 |
Journal | International Journal of Molecular Sciences |
Print ISSN | 1661-6596 |
Electronic ISSN | 1422-0067 |
Publisher | MDPI AG |
Peer Reviewed | Peer Reviewed |
Volume | 23 |
Issue | 1 |
Article Number | 126 |
DOI | https://doi.org/10.3390/ijms23010126 |
Keywords | Inorganic Chemistry; Organic Chemistry; Physical and Theoretical Chemistry; Computer Science Applications; Spectroscopy; Molecular Biology; General Medicine; Catalysis |
Public URL | https://nottingham-repository.worktribe.com/output/7108893 |
Publisher URL | https://www.mdpi.com/1422-0067/23/1/126 |
Files
ijms-23-00126
(2 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
You might also like
Editorial: In celebration of women in science: RNA networks and biology
(2023)
Journal Article
A virological view of tenascin-C in infection
(2022)
Journal Article
miR-155-3p: processing by-product or rising star in immunity and cancer?
(2022)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: digital-library-support@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2024
Advanced Search