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Objective assessment of blood and lymphatic vessel invasion and association with macrophage infiltration in cutaneous melanoma

Storr, Sarah J.; Safuan, Sabreena; Mitra, Angana; Elliott, Faye; Walker, Christopher; Vasko, Mark J.; Ho, Bernard; Cook, Martin; Mohammed, Rabab A.A.; Patel, Poulam M.; Ellis, Ian O.; Newton-Bishop, Julia A.; Martin, Stewart G.

Authors

Sarah J. Storr

Sabreena Safuan

Angana Mitra

Faye Elliott

Christopher Walker

Mark J. Vasko

Bernard Ho

Martin Cook

Rabab A.A. Mohammed

Poulam M. Patel

Ian O. Ellis

Julia A. Newton-Bishop

Stewart G. Martin

Abstract

The aims of this study were to investigate the role of vascular invasion (blood and lymphatic), vessel density and the presence of tumour-associated macrophages as prognostic markers in 202 cutaneous melanoma patients. Sections of primary melanoma were stained with lymphatic-specific antibody D2-40 to assess lymphatic vessel invasion and density in intratumoural and peritumoural areas; an antibody against endothelial marker CD34 was used to determine blood vessel invasion and density, and an antibody against CD68 was used to determine macrophage counts. Immunohistochemically determined vascular invasion (combined blood and lymphatic) was compared with that determined using haematoxylin and eosin (H&E) staining. The use of immunohistochemistry increased detection of vascular invasion from 8–30% of patients, and histological exam of H&E-stained tissue was associated with a false positive rate of 64%. Lymphatic vessel invasion occurred at a much higher frequency than blood vessel invasion (27 and 4% of patients, respectively). Although immunohistochemically detected vessel invasion was significantly associated with histological markers of adverse prognosis, such as increased Breslow thickness, ulceration and mitotic rate (all P

Journal Article Type Article
Publication Date Nov 11, 2011
Journal Modern Pathology
Print ISSN 0893-3952
Electronic ISSN 0893-3952
Publisher Nature Publishing Group
Peer Reviewed Peer Reviewed
Volume 25
Issue 4
Institution Citation Storr, S. J., Safuan, S., Mitra, A., Elliott, F., Walker, C., Vasko, M. J., …Martin, S. G. (2011). Objective assessment of blood and lymphatic vessel invasion and association with macrophage infiltration in cutaneous melanoma. Modern Pathology, 25(4), doi:10.1038/modpathol.2011.182
DOI https://doi.org/10.1038/modpathol.2011.182
Keywords D2-40, Melanoma, Tumour associated macrophages, Vascular invasion, Vessel density
Publisher URL http://www.nature.com/modpathol/journal/v25/n4/full/modpathol2011182a.html
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by-nc-nd/4.0




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