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Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Rosas-Magallanes, Vania; Stadthagen-Gomez, Gustavo; Rauzier, Jean; Barreiro, Luis B.; Tailleux, Ludovic; Boudou, Frédéric; Griffin, Ruth; Nigou, Jérome; Jackson, Mary; Gicquel, Brigittte; Neyrolles, Olivier

Authors

Vania Rosas-Magallanes

Gustavo Stadthagen-Gomez

Jean Rauzier

Luis B. Barreiro

Ludovic Tailleux

Frédéric Boudou

Jérome Nigou

Mary Jackson

Brigittte Gicquel

Olivier Neyrolles



Abstract

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

Citation

Rosas-Magallanes, V., Stadthagen-Gomez, G., Rauzier, J., Barreiro, L. B., Tailleux, L., Boudou, F., …Neyrolles, O. (2007). Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages. Infection and Immunity, 75(1), doi:10.1128/IAI.00058-06

Journal Article Type Article
Acceptance Date Sep 26, 2006
Online Publication Date Oct 9, 2006
Publication Date Jan 1, 2007
Deposit Date Dec 13, 2017
Publicly Available Date
Journal Infection and Immunity
Print ISSN 0019-9567
Electronic ISSN 1098-5522
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 75
Issue 1
DOI https://doi.org/10.1128/IAI.00058-06.
Public URL http://eprints.nottingham.ac.uk/id/eprint/48696
Publisher URL http://iai.asm.org/content/75/1/504
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf