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Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages

Rosas-Magallanes, Vania; Stadthagen-Gomez, Gustavo; Rauzier, Jean; Barreiro, Luis B.; Tailleux, Ludovic; Boudou, Frédéric; Griffin, Ruth; Nigou, Jérome; Jackson, Mary; Gicquel, Brigittte; Neyrolles, Olivier

Authors

Vania Rosas-Magallanes

Gustavo Stadthagen-Gomez

Jean Rauzier

Luis B. Barreiro

Ludovic Tailleux

Frédéric Boudou

Ruth Griffin mbzrg@nottingham.ac.uk

Jérome Nigou

Mary Jackson

Brigittte Gicquel

Olivier Neyrolles



Abstract

Using signature-tagged transposon mutagenesis, we isolated 23 Mycobacterium tuberculosis mutants, corresponding to 21 genes or genetic regions, attenuated in their ability to parasitize human macrophages. Mutants disrupted in the ABC transporter-encoding genes Rv0986 and Rv0987 were further characterized as being impaired in their ability to bind to host cells.

Journal Article Type Article
Publication Date Jan 1, 2007
Journal Infection and Immunity
Print ISSN 0019-9567
Electronic ISSN 1098-5522
Publisher American Society for Microbiology
Peer Reviewed Peer Reviewed
Volume 75
Issue 1
Institution Citation Rosas-Magallanes, V., Stadthagen-Gomez, G., Rauzier, J., Barreiro, L. B., Tailleux, L., Boudou, F., …Neyrolles, O. (2007). Signature-tagged transposon mutagenesis identifies novel mycobacterium tuberculosis genes involved in the parasitism of human macrophages. Infection and Immunity, 75(1), doi:10.1128/IAI.00058-06
DOI https://doi.org/10.1128/IAI.00058-06.
Publisher URL http://iai.asm.org/content/75/1/504
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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