APCI-MS/MS - An Enhanced Tool for the Real-Time Evaluation of Volatile Isobaric Compounds

Yang, Ni; Ford, Clive; Fisk, Ian

Abstract

Atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) can be used for the real-time analysis of volatile compounds both in vitro and in vivo. Recent developments of this technique, which are demonstrated herein, couple the APCI ion source and reaction chamber directly with tandem mass spectrometry (APCI-MS/MS). Nine aroma compounds were selected to demonstrate the benefit of the MS/MS approach using multiple reaction monitoring (MRM) mode compared to a single mass filtering step (single ion recording, SIR) in a static equilibrium system of corresponding aqueous solutions. The signal-to-noise ratio of this system operated in MRM mode was improved by a factor 1.5-25 compared with SIR mode. The MRM approach was also uniquely able to separate two isobaric aroma compounds (anisole and 2,5-dimethyl pyrazine), both in vitro (static headspace analysis) and in vivo (breath-by-breath). Overall, when APCI is coupled with tandem mass spectrometry, detection sensitivity was increased and isobaric compounds could be separated.

Citation

Yang, N., Ford, C., & Fisk, I. (2021). APCI-MS/MS - An Enhanced Tool for the Real-Time Evaluation of Volatile Isobaric Compounds. In J. D. Beauchamp (Ed.), Dynamic Flavor: Capturing Aroma Using Real-Time Mass Spectrometry (87-98). American Chemical Society. https://doi.org/10.1021/bk-2021-1402.ch007

• Online Publication Date: Nov 23, 2021
• Publication Date: Nov 23, 2021
• Deposit Date: Dec 2, 2021
• Publicly Available Date: Nov 24, 2024
• Publisher: American Chemical Society
• Pages: 87-98
• Series Title: ACS Symposium Series
• Series Number: 1402
• Series ISSN: 0097-6156
• Book Title: Dynamic Flavor: Capturing Aroma Using Real-Time Mass Spectrometry
• Chapter Number: 7
• ISBN: 9780841297944
• DOI: https://doi.org/10.1021/bk-2021-1402.ch007
• Public URL: https://nottingham-repository.worktribe.com/output/6847026
• Publisher URL: https://pubs.acs.org/doi/10.1021/bk-2021-1402.ch007