Esther Sweeney
An ex vivo cystic fibrosis model recapitulates key clinical aspects of chronic staphylococcus aureus infection
Sweeney, Esther; Harrington, Niamh E.; Harley Henriques, Alicia G.; Hassan, Marwa M.; Crealock-Ashurst, Branagh; Smyth, Alan R.; Hurley, Matthew N.; Tormo-Mas, Mar�a �ngeles; Harrison, Freya
Authors
Niamh E. Harrington
Alicia G. Harley Henriques
Marwa M. Hassan
Branagh Crealock-Ashurst
Alan R. Smyth
Matthew N. Hurley
Mar�a �ngeles Tormo-Mas
Freya Harrison
Abstract
Staphylococcus aureus is the most prevalent organism isolated from the airways of people with cystic fibrosis (CF), predomi-nantly early in life. Yet its role in the pathology of lung disease is poorly understood. In mice, and many experiments using cell lines, the bacterium invades cells or interstitium, and forms abscesses. This is at odds with the limited available clinical data: interstitial bacteria are rare in CF biopsies and abscesses are highly unusual. Bacteria instead appear to localize in mucus plugs in the lumens of bronchioles. We show that, in an established ex vivo model of CF infection comprising porcine bronchiolar tissue and synthetic mucus, S. aureus demonstrates clinically significant characteristics including colonization of the airway lumen, with preferential localization as multicellular aggregates in mucus, initiation of a small colony variant phenotype and increased antibiotic tolerance of tissue-associated aggregates. Tissue invasion and abscesses were not observed. Our results may inform ongoing debates relating to clinical responses to S. aureus in people with CF.
Citation
Sweeney, E., Harrington, N. E., Harley Henriques, A. G., Hassan, M. M., Crealock-Ashurst, B., Smyth, A. R., …Harrison, F. (2021). An ex vivo cystic fibrosis model recapitulates key clinical aspects of chronic staphylococcus aureus infection. Microbiology, 167(1), Article 000987. https://doi.org/10.1099/mic.0.000987
Journal Article Type | Article |
---|---|
Acceptance Date | Oct 12, 2020 |
Online Publication Date | Nov 13, 2020 |
Publication Date | 2021-01 |
Deposit Date | Dec 29, 2020 |
Publicly Available Date | Mar 28, 2024 |
Journal | Microbiology |
Print ISSN | 1350-0872 |
Electronic ISSN | 1465-2080 |
Publisher | Microbiology Society |
Peer Reviewed | Peer Reviewed |
Volume | 167 |
Issue | 1 |
Article Number | 000987 |
DOI | https://doi.org/10.1099/mic.0.000987 |
Keywords | Microbiology |
Public URL | https://nottingham-repository.worktribe.com/output/5040311 |
Publisher URL | https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000987 |
Additional Information | © Niamh E. Harrington, Alicia G. Harley Henriques, Marwa M. Hassan, Branagh Crealock- Ashurst, Alan R. Smyth, Matthew N. Hurley, María Ángeles Tormo- Mas and Freya Harrison, 2020. The definitive peer reviewed, edited version of this article is published in [Micrbiology, 167, 1, 2021, https://doi.org/10.1099/mic.0.000987 |
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