Carbamazepine induces a bioenergetics disruption to microvascular endothelial cells from the blood-brain barrier

Alelwani, Walla; Elmorsy, Ekramy; Kattan, Shahad W.; Babteen, Nouf Abubakr; Alnajeebi, Afnan M.; Al-Ghafari, Ayat; Carter, Wayne G.

Abstract

Carbamazepine (CBZ) is a widely employed anti-seizure medication that crosses the blood-brain barrier (BBB) to exert its anti-convulsant action. The effects of CBZ on components of the BBB have yet to be completely delineated. Hence the current study evaluated the effects of CBZ upon mitochondrial functionality of BBB-derived microvascular endothelial cells isolated from Albino rats. The influence of CBZ on cell viability and barrier functions were evaluated by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), lactate dehydrogenase, and electrophysiological assays over a drug concentration range of 0.1-1000 µM. Bioenergetics effects were measured via ATP production, mitochondrial complexes I and

Citation

Alelwani, W., Elmorsy, E., Kattan, S. W., Babteen, N. A., Alnajeebi, A. M., Al-Ghafari, A., & Carter, W. G. (2020). Carbamazepine induces a bioenergetics disruption to microvascular endothelial cells from the blood-brain barrier. Toxicology Letters, 333, 184-191. https://doi.org/10.1016/j.toxlet.2020.08.006

• Journal Article Type: Article
• Acceptance Date: Aug 11, 2020
• Online Publication Date: Aug 14, 2020
• Publication Date: Oct 15, 2020
• Deposit Date: Aug 12, 2020
• Publicly Available Date: Aug 15, 2021
• Journal: Toxicology Letters
• Print ISSN: 0378-4274
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 333
• Pages: 184-191
• DOI: https://doi.org/10.1016/j.toxlet.2020.08.006
• Keywords: Toxicology; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/4828350
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S0378427420303969