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Carbamazepine induces a bioenergetics disruption to microvascular endothelial cells from the blood-brain barrier

Alelwani, Walla; Elmorsy, Ekramy; Kattan, Shahad W.; Babteen, Nouf Abubakr; Alnajeebi, Afnan M.; Al-Ghafari, Ayat; Carter, Wayne G.

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Authors

Walla Alelwani

Ekramy Elmorsy

Shahad W. Kattan

Nouf Abubakr Babteen

Afnan M. Alnajeebi

Ayat Al-Ghafari



Abstract

Carbamazepine (CBZ) is a widely employed anti-seizure medication that crosses the blood-brain barrier (BBB) to exert its anti-convulsant action. The effects of CBZ on components of the BBB have yet to be completely delineated. Hence the current study evaluated the effects of CBZ upon mitochondrial functionality of BBB-derived microvascular endothelial cells isolated from Albino rats. The influence of CBZ on cell viability and barrier functions were evaluated by 3-(4,5 dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), lactate dehydrogenase, and electrophysiological assays over a drug concentration range of 0.1-1000 µM. Bioenergetics effects were measured via ATP production, mitochondrial complexes I and

Citation

Alelwani, W., Elmorsy, E., Kattan, S. W., Babteen, N. A., Alnajeebi, A. M., Al-Ghafari, A., & Carter, W. G. (2020). Carbamazepine induces a bioenergetics disruption to microvascular endothelial cells from the blood-brain barrier. Toxicology Letters, 333, 184-191. https://doi.org/10.1016/j.toxlet.2020.08.006

Journal Article Type Article
Acceptance Date Aug 11, 2020
Online Publication Date Aug 14, 2020
Publication Date Oct 15, 2020
Deposit Date Aug 12, 2020
Publicly Available Date Mar 28, 2024
Journal Toxicology Letters
Print ISSN 0378-4274
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 333
Pages 184-191
DOI https://doi.org/10.1016/j.toxlet.2020.08.006
Keywords Toxicology; General Medicine
Public URL https://nottingham-repository.worktribe.com/output/4828350
Publisher URL https://www.sciencedirect.com/science/article/abs/pii/S0378427420303969