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The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer Rhodobacter sphaeroides is regulated by the global Prr pathway

Jeong, E.-L.; Broad, S.J.; Moody, R.G.; Phillips-Jones, M.K.

The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer Rhodobacter sphaeroides is regulated by the global Prr pathway Thumbnail


Authors

E.-L. Jeong

S.J. Broad

R.G. Moody

M.K. Phillips-Jones



Abstract

Expression of fdp, encoding a fasciclin I domain protein important for adherence in the hydrogenproducing bacterium Rhodobacter sphaeroides, was investigated under a range of conditions to gain insights into optimization of adherence for immobilization strategies suitable for H2 production. The fdp promoter was linked to a lacZ reporter and expressed in wild type and in PRRB and PRRA mutant strains of the Prr regulatory pathway. Expression was significantly negatively regulated by Prr under all conditions of aerobiosis tested including anaerobic conditions (required for H2 production), and aerobically regardless of growth phase, growth medium complexity or composition, carbon source, heat and cold shock and dark/light conditions. Negative fdp regulation by Prr was reflected in cellular levels of translated Fdp protein. Since Prr is required directly for nitrogenase expression, we propose optimization of Fdp-based adherence in R. sphaeroides for immobilised biohydrogen production by inactivation of the PrrA binding site(s) upstream of fdp.

Citation

Jeong, E., Broad, S., Moody, R., & Phillips-Jones, M. (2020). The adherence-associated Fdp fasciclin I domain protein of the biohydrogen producer Rhodobacter sphaeroides is regulated by the global Prr pathway. International Journal of Hydrogen Energy, 45(51), 26840-26854. https://doi.org/10.1016/j.ijhydene.2020.07.108

Journal Article Type Article
Acceptance Date Jul 12, 2020
Online Publication Date Aug 7, 2020
Publication Date Oct 16, 2020
Deposit Date Jul 16, 2020
Publicly Available Date Mar 29, 2024
Journal International Journal of Hydrogen Energy
Print ISSN 0360-3199
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 45
Issue 51
Pages 26840-26854
DOI https://doi.org/10.1016/j.ijhydene.2020.07.108
Keywords Fuel Technology; Renewable Energy, Sustainability and the Environment; Energy Engineering and Power Technology; Condensed Matter Physics
Public URL https://nottingham-repository.worktribe.com/output/4769662
Publisher URL https://www.sciencedirect.com/science/article/pii/S0360319920326835

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