Electronic cigarette vapour moderately stimulates pro-inflammatory signalling pathways and interleukin-6 production by human monocyte-derived dendritic cells

Chen, I. Ling; Todd, Ian; Tighe, Patrick J.; Fairclough, Lucy C.

Abstract

© 2020, The Author(s). Dendritic cells (DCs) are professional antigen presenting cells that play a critical role in bridging innate and adaptive immunity. Numerous studies have shown that tobacco constituents present in conventional cigarettes affect the phenotype and function of DCs; however, no studies have examined the effects of vapour from E-cigarettes on human DCs. Here, the effects of E-cigarette vapour extract (ECVE) on the phenotype and function of DCswere investigated by creating an in vitro cell culture model using human monocyte-derived DCs (MoDCs). Immature DCs were generated from peripheral blood monocytes and mature DCs were then produced by treatment with LPS or Poly I:C for 24h. For LPS-matured DCs, 3% ECVE treatment slightly suppressed HLA-DR and CD86 expression, whereas 1% ECVE treatment enhanced IL-6 production. The overall expression of 29 signalling molecules and other cytoplasmic proteins (mainly associated with DC activation) was significantly upregulated in immature DCs by 1% ECVE, and in LPS-treated DCs by 3% ECVE. In particular, the condition that induced IL-6 production also upregulated MAPK pathway activation. These findings indicate that E-cigarette vapour moderately affects human DCs, but the effects are less pronounced than those reported for tobacco smoke.

Citation

Chen, I. L., Todd, I., Tighe, P. J., & Fairclough, L. C. (2020). Electronic cigarette vapour moderately stimulates pro-inflammatory signalling pathways and interleukin-6 production by human monocyte-derived dendritic cells. Archives of Toxicology, 94(6), 2097-2112 . https://doi.org/10.1007/s00204-020-02757-8

• Journal Article Type: Article
• Acceptance Date: Apr 21, 2020
• Online Publication Date: May 5, 2020
• Publication Date: 2020-06
• Deposit Date: Apr 23, 2020
• Publicly Available Date: May 6, 2021
• Journal: Archives of Toxicology
• Print ISSN: 0340-5761
• Electronic ISSN: 1432-0738
• Publisher: Springer Verlag
• Peer Reviewed: Peer Reviewed
• Volume: 94
• Issue: 6
• Pages: 2097-2112
• DOI: https://doi.org/10.1007/s00204-020-02757-8
• Keywords: Toxicology; Health, Toxicology and Mutagenesis; General Medicine
• Public URL: https://nottingham-repository.worktribe.com/output/4329121
• Publisher URL: https://link.springer.com/article/10.1007%2Fs00204-020-02757-8
• Additional Information: Received: 9 April 2020; Accepted: 21 April 2020; First Online: 5 May 2020; : ; : The authors state that they have no conflicts of interest to declare.