Cas3 Protein—A Review of a Multi-Tasking Machine

He, Liu; St. John James, Michael; Radovcic, Marin; Ivancic-Bace, Ivana; Bolt, Edward L.

Abstract

Cas3 has essential functions in CRISPR immunity but its other activities and roles, in vitro and in cells, are less widely known. We offer a concise review of the latest understanding and questions arising from studies of Cas3 mechanism during CRISPR immunity, and highlight recent attempts at using Cas3 for genetic editing. We then spotlight involvement of Cas3 in other aspects of cell biology, for which understanding is lacking—these focus on CRISPR systems as regulators of cellular processes in addition to defense against mobile genetic elements

Citation

He, L., St. John James, M., Radovcic, M., Ivancic-Bace, I., & Bolt, E. L. (2020). Cas3 Protein—A Review of a Multi-Tasking Machine. Genes, 11(2), Article 208. https://doi.org/10.3390/genes11020208

• Journal Article Type: Article
• Acceptance Date: Feb 16, 2020
• Online Publication Date: Feb 18, 2020
• Publication Date: Feb 18, 2020
• Deposit Date: Aug 17, 2020
• Publicly Available Date: Aug 18, 2020
• Journal: Genes
• Print ISSN: 2073-4425
• Electronic ISSN: 2073-4425
• Publisher: MDPI
• Peer Reviewed: Peer Reviewed
• Volume: 11
• Issue: 2
• Article Number: 208
• DOI: https://doi.org/10.3390/genes11020208
• Keywords: Genetics(clinical); Genetics
• Public URL: https://nottingham-repository.worktribe.com/output/4106370
• Publisher URL: https://www.mdpi.com/2073-4425/11/2/208