Relaxin signalling in THP-1 cells uses a novel phosphotyrosine-dependent pathway

Anand-Ivell, Ravinder; Heng, Kee; Bartsch, Olaf; Ivell, Richard

Abstract

The heterodimeric peptide hormone relaxin acts through the novel G-protein coupled receptor LGR7 to elicit the production of cAMP in the human monocyte cell line THP-1. The very small number of receptors on the cell surface, and the lack of response in cell membranes imply the involvement of a cytoplasmic signal amplification process. Here we show that this process comprises a novel and specific tyrosine kinase activity close to the receptor, and involves neither protein kinase A, mitogen-activated protein kinase, nor phosphoinositide-3 kinase activities as major upstream components. Furthermore, this novel involvement of a tyrosine kinase activity is cell-type dependent, being largely absent from LGR7-transfected HEK293T cells, and receptor-dependent; vasoactive intestinal peptide or isoproterenol signalling in the same cells does not require this tyrosine kinase activity. © 2007 Elsevier Ireland Ltd. All rights reserved.

Citation

Anand-Ivell, R., Heng, K., Bartsch, O., & Ivell, R. (2007). Relaxin signalling in THP-1 cells uses a novel phosphotyrosine-dependent pathway. Molecular and Cellular Endocrinology, 272(1-2), 1-13. https://doi.org/10.1016/j.mce.2007.04.001

• Journal Article Type: Article
• Acceptance Date: Apr 2, 2007
• Online Publication Date: Apr 6, 2007
• Publication Date: Jun 30, 2007
• Deposit Date: Jun 15, 2021
• Journal: Molecular and Cellular Endocrinology
• Print ISSN: 0303-7207
• Electronic ISSN: 1872-8057
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 272
• Issue: 1-2
• Pages: 1-13
• DOI: https://doi.org/10.1016/j.mce.2007.04.001
• Public URL: https://nottingham-repository.worktribe.com/output/3176604
• Publisher URL: https://www.sciencedirect.com/science/article/abs/pii/S0303720707001505?via%3Dihub